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I5635

Sigma-Aldrich

Monoclonal Anti-Human IgG2 antibody produced in mouse

clone HP-6014, ascites fluid

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Synonym(s):
Monoclonal Anti-Human IgG2
NACRES:
NA.46

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6014, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:5,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
I2513I5885I6510
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

isotype

IgG1

isotype

IgG2a

isotype

IgG1

isotype

IgG2b

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

HP-6014, monoclonal

clone

8c/6-39, monoclonal

clone

GG-5, monoclonal

clone

GE-1, monoclonal

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General description

Monoclonal Anti-Human IgG2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Human IgG consists of four subclasses.

Specificity

The antibody is specific for human IgG2 and is non-reactive with other IgG subclasses. This clone has been singled out as the most widely applicable IgG2 specific monoclonal antibody by the IUIS/WHO study.

Application

Monoclonal Anti-Human IgG2 antibody has been used in in enzyme linked immunosorbent assay (ELISA) and in western blotting.
It may also be used:
  • in imprint immunofixation
  • in immunofluorometric assay
  • in hemagglutination
  • in hemagglutination inhibition
  • in particle counting immunoassay
  • in detection of cytoplasmic IgG

Biochem/physiol Actions

Anti-polysaccharide responses are mainly of the IgG2 sub-class while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccharides stimulate an IgG2 response in peripheral blood leukocytes (PBL′s).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Identification of immune biomarkers related to disease progression and treatment efficacy in human visceral leishmaniasis
Portela A S B, et al.
Immunobiology, 223(3), 303-309 (2018)
A conserved Leishmania hypothetical protein evaluated for the serodiagnosis of canine and human visceral and tegumentary leishmaniasis, as well as a serological marker for the posttreatment patient follow-up
Ribeiro P A, et al.
Diagnostic Microbiology and Infectious Disease, 92(3), 196-203 (2018)
Negative immunodiffusion test results obtained with sera of paracoccidioidomycosis patients may be related to low-avidity immunoglobulin G2 antibodies directed against carbohydrate epitopes
Neves AR, et al.
Clinical and Diagnostic Laboratory Immunology, 10(5), 802-807 (2003)
Z Lazarova et al.
The Journal of investigative dermatology, 114(1), 178-184 (2000-01-05)
Patients with one form of cicatricial pemphigoid have IgG antibasement membrane autoantibodies against laminin 5 (alpha3beta3gamma2). Although passive transfer of rabbit anti-laminin 5 IgG to neonatal mice has been shown to induce subepidermal blisters that mimic those in patients, it
D Thiagarajan et al.
ACR open rheumatology, 2(6), 344-356 (2020-05-12)
Immunoglobulin M antibodies against phosphorylcholine (anti-PCs) may be protective in atherosclerosis, cardiovascular disease (CVD), and systemic lupus erythematosus (SLE). We study immunoglobulin G1 (IgG1) and immunoglobulin G2 (IgG2) anti-PCs, with a focus on atherosclerosis and SLE. We determined anti-PCs by

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