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I8135

Sigma-Aldrich

IgM from bovine serum

reagent grade, ≥95% (SDS-PAGE or HPLC), buffered aqueous solution

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Synonym(s):
Bovine IgM
MDL number:

conjugate

unconjugated

grade

reagent grade

Assay

≥95% (SDS-PAGE or HPLC)

form

buffered aqueous solution

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
I8260I9640M5170
IgM from bovine serum reagent grade, ≥95% (SDS-PAGE or HPLC), buffered aqueous solution

Sigma-Aldrich

I8135

IgM from bovine serum

IgM from human serum reagent grade, ~95% (HPLC), buffered aqueous solution

Sigma-Aldrich

I8260

IgM from human serum

Sigma-Aldrich

Sigma-Aldrich

I9640

IgG from bovine serum

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

grade

reagent grade

grade

reagent grade

grade

technical grade

grade

-

assay

≥95% (SDS-PAGE or HPLC)

assay

~95% (HPLC)

assay

≥80% (SDS-PAGE)

assay

-

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
IgM from bovine serum is purified from normal bovine serum by precipitation and gel filtration techniques.
IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It exists as a pentamer and is secreted by peritoneal B cells.

Application

IgM from bovine serum has been used for screening the lipopolysaccharide levels in Limulus amoebocyte lysate (LAL) assay. IgM has been used in flow cytometry assay for detecting FcγRIV expression and immunoglobulin binding.
Purified bovine IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.

Biochem/physiol Actions

IgM plays a key role in engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.

Physical form

Solution in 0.05 M Tris-HCl, 0.15 M sodium chloride and 0.01 M glycine, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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