Skip to Content
MilliporeSigma
All Photos(1)

Documents

L1412

Sigma-Aldrich

Lysing Enzymes from Trichoderma harzianum

lyophilized powder

Synonym(s):

Glucanex®

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352204

form

lyophilized powder

specific activity

≥10 units/g

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

Application

Lysing Enzymes from Trichoderma harzianum is suitable retrieve DNA plugs from agarose gels. It has been used:
  • for protoplast isolation
  • for hydrolyzing poly(1-3)-glucose of the yeast cell wall glucan prior to yeast spheroplast transformation
  • for the lysis of Aspergillus niger vegetative cells

Biochem/physiol Actions

Lysing Enzymes from Trichoderma harzianum is called Glucanex. It comprised of mixture of isoenzymes with β-1, 3 glucanase activity. Lysing Enzymes from Trichoderma harzianum hydrolyzes yeast cell wall oligosaccharides to glucan for protoplast preparation. Its stability is influenced by pH changes.

Quality

Contains β-glucanase, cellulase, protease, and chitinase activities

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at ph 7.5 at 37 deg C (color byfolin-ciocalteu reagent).

Legal Information

A product of Novozyme Corp.
Glucanex is a registered trademark of Novozymes Corp.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Thomas Leisen et al.
PLoS pathogens, 16(8), e1008326-e1008326 (2020-08-18)
CRISPR/Cas has become the state-of-the-art technology for genetic manipulation in diverse organisms, enabling targeted genetic changes to be performed with unprecedented efficiency. Here we report on the first establishment of robust CRISPR/Cas editing in the important necrotrophic plant pathogen Botrytis
Divya Ramalingam Iyer et al.
PLoS genetics, 13(8), e1006958-e1006958 (2017-08-15)
In response to DNA damage during S phase, cells slow DNA replication. This slowing is orchestrated by the intra-S checkpoint and involves inhibition of origin firing and reduction of replication fork speed. Slowing of replication allows for tolerance of DNA
Xiao-Lian Yuan et al.
Molecular genetics and genomics : MGG, 279(6), 545-561 (2008-03-06)
The filamentous ascomycete Aspergillus niger is well known for its ability to produce a large variety of enzymes for the degradation of plant polysaccharide material. A major carbon and energy source for this soil fungus is starch, which can be
Cylindrical cellular geometry ensures fidelity of division site placement in fission yeast
Mishra M, et al.
Journal of Cell Science, 125(16), 3850-3857 (2012)
Influence of protoplast fusion between two Trichoderma spp. on extracellular enzymes production and antagonistic activity
Hassan MM
Biotechnology, Biotechnological Equipment, 28(6), 1014-1023 (2014)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service