L3272
LB Broth with agar (Luria low salt)
Powder microbial growth medium
Synonym(s):
Luria Agar Base (Miller’s Modification)
About This Item
Recommended Products
grade
for molecular biology
Quality Level
sterility
non-sterile
form
powder
composition
Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(3.05% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
Related Categories
General description
Application
- for culturing E. coli DH10B cells.
- in the isolation of diazotrophic bacteria.
- for transformation of competent E. coli strain Stbl3 for cloning.
Features and Benefits
- Easy scale-up using larger package sizes
- A budget-friendly alternative to pre-poured plates
- Standard formulation
Preparation Note
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.
Legal Information
related product
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Protocols
General protocols for growth of competent cells in microbial medium.
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