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L4895

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

FITC conjugate, lyophilized powder

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Synonym(s):
WGA, Wheat germ agglutinin
NACRES:
NA.32

conjugate

FITC conjugate

Quality Level

form

lyophilized powder

potency

<50 μg/mL agglutination activity

composition

Protein, ~50% Lowry

extent of labeling

~2 mol FITC per mol protein

storage temp.

2-8°C

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General description

Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.

Application

For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Lectin from Triticum vulgaris (wheat) has been used:
  • to treat transfected cells for immunofluorescence
  • to stereotaxically inject into mice for neuronal tracing
  • in immunohistochemistry for the analysis of myocytes

Biochem/physiol Actions

Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

Package size based on protein content.

Physical form

Contains phosphate buffer salts

Preparation Note

Conjugates are prepared from affinity purified lectin.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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