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L9006

Sigma-Aldrich

Lectin from Ulex europaeus (gorse, furze)

FITC conjugate, lyophilized powder

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Synonym(s):
Ulex europaeus agglutinin, UEA-I
NACRES:
NA.32

conjugate

FITC conjugate

Quality Level

form

lyophilized powder

potency

<5 μg/mL agglutination activity

composition

Protein, ~10% Lowry

extent of labeling

2-5 mol FITC per mol protein

storage temp.

−20°C

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Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Packaging

Package size based on protein content.

Linkage

Conjugates are prepared from affinity purified lectin.

Physical form

Contains potassium phosphate buffer salts and NaCl

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Articles

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