MilliporeSigma
All Photos(1)

Documents

L0770

Sigma-Aldrich

Lectin from Pisum sativum (pea)

FITC conjugate, lyophilized powder

Sign Into View Organizational & Contract Pricing

Synonym(s):
Pisum sativum agglutinin, PSA
NACRES:
NA.32

conjugate

FITC conjugate

Quality Level

form

lyophilized powder

potency

<20 μg per mL agglutination activity

composition

Protein, ≥95% biuret

extent of labeling

2-4 mol FITC per mol protein (Lowry).

impurities

salt, free

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
L6512L5380L2769
Lectin from Pisum sativum (pea) FITC conjugate, lyophilized powder

L0770

Lectin from Pisum sativum (pea)

Sigma-Aldrich

L6512

Lectin from Helix pomatia

Lectin from Pisum sativum (pea) lyophilized powder

L5380

Lectin from Pisum sativum (pea)

impurities

salt, free

impurities

salt, free

impurities

salt, free

impurities

salt, free

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

potency

<20 μg per mL agglutination activity

potency

-

potency

<20 μg/mL per mL agglutination activity

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.), ≤1.0 μg/mL leucoagglutination activity, ≤5 μg/mL mitogenic activity

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

FITC conjugate

conjugate

biotin conjugate

conjugate

-

conjugate

-

General description

Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for α-D-glucose and α-D-mannose.

Application

Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns

Biochem/physiol Actions

Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A).
PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.

Preparation Note

Conjugates are prepared from affinity purified lectin.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Tácia Gomes Bergstein-Galan et al.
Theriogenology, 96, 69-75 (2017-05-24)
This study investigates the effect of time of storage of epididymides at room temperature and the addition of 20% of seminal plasma to the cryopreservation extender, on post thaw quality and fertility of ovine spermatozoa collected from the cauda epididymis.
L S Castro et al.
Journal of animal science and biotechnology, 7, 17-17 (2016-03-08)
In order to improve the efficiency of bovine sperm cryopreservation process, it is important to understand how spermatozoa respond to differences in temperature as well as the ability to recover its own metabolism. The combination between flow cytometry approach and
Gediendson Ribeiro de Araujo et al.
Animal reproduction science, 163, 56-62 (2015-11-04)
We evaluated the capacity of ocelot and oncilla spermatozoa to bind to the perivitelline membranes (PVMs) of hen eggs in a sperm binding assay (S-PVM). In addition, a device that improves the standardization of the assay was developed. The number
The biological significance of phospholipase C beta 1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development.
Choi D, et al.
Journal of Assisted Reproduction and Genetics, 18(5), 305-310 (2001)
In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen
Aires VA, et al.
Theriogenology, 60(2), 269-279 (2003)

Articles

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service