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L6135

Sigma-Aldrich

Lectin from Arachis hypogaea (peanut)

biotin conjugate, lyophilized powder

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Synonym(s):
PNA, Peanut agglutinin
NACRES:
NA.32

conjugate

biotin conjugate

Quality Level

form

lyophilized powder

mol wt

120 kDa

composition

Protein, ≥80% Lowry

extent of labeling

~3 mol biotin per mol protein

technique(s)

blood typing: suitable
fractionation: suitable

solubility

water: 1.00-1.10 mg/mL, clear to slightly hazy, colorless

storage temp.

2-8°C

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General description

Peanut lectin (PNA) is a lectin or an agglutinin from the legume, Arachis hypogaea, found in peripheral venous blood in humans after ingestion of peanuts. PNA lectin is specific to the galactosyl (β-1,3) N-acetylgalactosamine.

Application

Lectin from Arachis hypogaea (peanut) has been used:
  • in enzyme-linked micro plate assay to detect β-D-galactose-N-acetylglucosamine
  • in Immunohistochemical double staining
  • Immunohistochemical double staining for the detection of germinal centers
  • in enzyme-linked microplate assayto detect β-D-galactose-N-acetylglucosamine

Biochem/physiol Actions

Lectin from Arachis hypogaea (peanut) functions as an antigenic determinant for Thomsen-Friedenreich antigen (TF) and as mitogen for colorectal cancer stimulation cell division in normal colonic epithelium. It is therefore used as tumor marker.
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.

Packaging

Package size based on protein content.

Physical form

Contains sodium citrate

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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