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L7395

Sigma-Aldrich

Lipoxidase from Glycine max (soybean)

Type I-B, lyophilized powder, ≥50,000 units/mg solid

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Synonym(s):
15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Type I-B

Quality Level

form

lyophilized powder

specific activity

≥50,000 units/mg solid

mol wt

94 kDa

shipped in

wet ice

storage temp.

−20°C

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General description

Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.

Application

Lipoxidase from Glycine max (soybean) has been used:
  • to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins
  • as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives
  • as a positive control in lipoxygenase inhibitory activity assay

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Biochem/physiol Actions

Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Unit Definition

One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Physical form

Contains stabilizer and NaCl

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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