L7651
Lysozyme from chicken egg white
lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein, for molecular biology
Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
About This Item
Recommended Products
grade
for molecular biology
form
lyophilized powder
specific activity
≥40,000 units/mg protein
mol wt
single-chain 14.3 kDa
composition
protein, ≥90%
UniProt accession no.
storage temp.
−20°C
Gene Information
chicken ... LYZ(396218)
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Application
Biochem/physiol Actions
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Features and Benefits
- Highly purified by repeated crystallization and dialysis
- Each lot is use-tested for isolation of plasmid DNA from E. coli
Unit Definition
Physical form
Preparation Note
Replaced by
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How can I reconstitute Lysozyme from chicken egg white, Product L7651?
For E. coli cell lysis, use a freshly prepared lysozyme solution (10 mg/mL) in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/mL) yielding a clear to slightly hazy colorless solution.
How stable are solutions of Lysozyme from chicken egg white, Product L7651?
Aqueous solutions should retain activity for at least one month when stored between 2-8 °C.
Is Lysozyme from chicken egg white, Product L7651, suitable for bacterial lysis prior to DNA purification?
Each lot is verified to be suitable for use as a lysing agent in purifying plasmid DNA from E. coli. You can also use this product in combination with the GenElute™ Bacterial Genomic DNA Kit (Product No. NA2100) to purify genomic DNA from Gram-positive bacteria.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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