M047030

Cyanine 3 Phosphoramidite

configured for ABI

• Synonym(s): 2-[3-[1,3-Dihydro-1-[3-(4-methoxytriphenylmethoxy)propyl]-3,3-dimethyl-2H-indol-2-ylidene]-1-propen-1-yl]-1-[3-[N,N-diisopropylamino(2-cyanoethoxy)phosphinoxy]propyl]-3,3-dimethyl-3H-indolium
• Empirical Formula (Hill Notation): C₅₈H₇₀N₄O₄P
• CAS Number: 718594-66-2
• Molecular Weight: 918.17
• UNSPSC Code: 41116105
• NACRES: NA.21

Properties

• product line: Proligo Reagents
• Quality Level: 200
• assay: ≥90% (³¹P-NMR); ≥95.0% (@549nm, reversed phase HPLC)
• form: solid
• mol wt: 953.64 g/mol
• technique(s): DNA synthesis: suitable
• color: dark red, 0.1M in Acetonitrile
• turbidity: clear
• solubility: acetonitrile: 0.1 M, clear, dark red
• λ: conforms (UV/VIS Identity)
• compatibility: configured for ABI
• storage temp.: −70°C

Description

General description

Phosphoramidite technology is ideally suited for the modification ofsynthetic oligonucleotides with covalently attached reporter moieties,linkers, spacers or haptens. Sigma-Aldrich provides popular reporters andlinkers ready to use for DNA/RNA synthesis machines such as β-cyanoethylphosphoramidites.

Application

Cyanine 3 Phosphoramidite is designated for incorporation (internally) within oligonucleotides during automated DNA synthesis. It has been used in Fluorescence labeling and imaging studies.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable