MilliporeSigma
All Photos(1)

Documents

M3821

Sigma-Aldrich

Anti-Myelin Basic Protein (MBP) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-MBP
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 17-22 kDa (isoform)
antigen ~22 kDa (isoform)
antigen ~33 kDa (isoform)

species reactivity

rat, human, mouse

concentration

~1 mg/mL

technique(s)

flow cytometry: 1:200-1:500
immunohistochemistry: 1:100-1:200
western blot: 1:500-1:1,000

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MBP(4155)
mouse ... Mbp(17196)
rat ... Mbp(24547)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
M9434MAB382ABN912
Anti-Myelin Basic Protein from rabbit, purified by affinity chromatography

ABN912

Anti-Myelin Basic Protein

antibody form

affinity isolated antibody

antibody form

tissue culture supernatant

antibody form

culture supernatant

antibody form

affinity isolated antibody

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

mol wt

antigen 17-22 kDa (isoform), antigen ~33 kDa (isoform), antigen ~22 kDa (isoform)

mol wt

-

mol wt

-

mol wt

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

-

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Myelin Basic Protein (MBP) is a hydrophilic protein that is expressed mainly in oligodendrocytes. It has a pivotal role in myelination of nervous system, signaling pathways prior to myelination and remyelination processes after neural injury. Anti-myelin basic protein (MBP) antibody can be used in western blotting and flow cytometry. Rabbit anti-myelin basic protein antibody reacts specifically with myelin basic protein of mouse, rat and human.
Myelin basic protein (MBP) is an 18.5kDa protein encoded by the gene mapped to human chromosome 18q22qter. MBP is a major component of the myelin sheath and is highly expressed in central nervous system (CNS).

Immunogen

synthetic peptide corresponding to residues 102-116 of human Myelin Basic Protein.

Application

Anti-Myelin Basic Protein (MBP) antibody produced in rabbit has been used in:
  • indirect immunolabeling
  • immunohistochemistry.
  • Western Blotting.
Anti-myelin basic protein (MBP) antibody (diluted 1:200) can be used as a primary antibody in double fluorescence labeling. It can also be used to detect myelin basic protein using immunoblotting.

Biochem/physiol Actions

Myelin basic protein (MBP) plays a vital role in formation of myelin sheath and is associated with the various neurological disorders. MBP functions as an antigenic target in autoimmune disease experimental allergic encephalomyelitis (EAE). MBP plays an essential role in cytoskeletal turnover and membrane extension. Additionally, it is also involved in Fyn-mediated signaling pathways, sequestration of phosphoinositides and regulation of calcium homoeostasis. MBP functions as an in vitro substrate for mitogen-activated protein kinase (MAP) kinase and aids in the purification and functional studies of enzyme.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Identification by mass spectrometry of threonine 97 in bovine myelin basic protein as a specific phosphorylation site for mitogen-activated protein kinase.
Erickson AK, et al.
The Journal of Biological Chemistry, 265, 19728-19735 (1990)
Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerve.
Nishimoto S, et al.
Frontiers in Cellular Neuroscience (2015)
Neurotropin? Accelerates the Differentiation of Schwann Cells and Remyelination in a Rat Lysophosphatidylcholine-Induced Demyelination Model
Matsuoka H, et al.
International Journal of Molecular Sciences, 19 (2018)
MyelStones: the executive roles of myelin basic protein in myelin assembly and destabilization in multiple sclerosis.
Vassall KA, et al.
The Biochemical Journal, 472, 17-32 (2015)
Neuroglial Cells in Long-Term Primary Cultures from the Gilthead Seabream (Sparus Aurata L.): New Functional In Vitro Model from Bony Fish Brain
Centoducati, et al.
Italian Journal of Animal Science, 12 (2013)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service