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Aldehyde Dehydrogenase Activity Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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ALDH Activity Assay Kit


sufficient for 100 colorimetric tests

detection method


relevant disease(s)

cardiovascular diseases; cancer; hematological disorder

storage temp.


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detection method


detection method


detection method


detection method


storage temp.


storage temp.


storage temp.


storage temp.


relevant disease(s)

cardiovascular diseases; cancer; hematological disorder

relevant disease(s)


relevant disease(s)

hematological disorder; cancer

relevant disease(s)

gastrointestinal diseases; cardiovascular diseases

General description

Aldehyde dehydrogenases (ALDH) are a family of enzymes that catalyze the metabolism of exogenous and endogenous aldehydes, preventing the accumulation of potentially reactive and toxic aldehydes and their metabolites. In addition to their role in aldehyde metabolism, ALDH enzymes also play critical roles in other cellular processes such as cell proliferation, differentiation, and survival. Mutations in ALDH family members have been linked to numerous diseases such as heart disease, cataracts, and certain cancers. ALDH activity may also serve as a marker for certain stem cell populations including hematopoietic stem cells and certain cancer stem cells.


Aldehyde Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the activity of aldehyde dehydrogenase.

Features and Benefits

Compatible with high-throughput handling systems.


Suitable for the measurement of aldehyde dehydrogenase (ALDH) in a variety of samples including tissue and cells


The Aldehyde Dehydrogenase Activity Colorimetric Assay kit provides a simple and direct procedure for measuring NAD-dependent ALDH activity in a variety of samples. ALDH activity is determined by a coupled enzyme assay in which acetaldehyde is oxidized by ALDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the ALDH activity present. One unit of ALDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.


CorrosionHealth hazard

Signal Word


Hazard Statements

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



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Aldehyde Dehydrogenase Inhibitors: a Comprehensive Review of the Pharmacology, Mechanism of Action, Substrate Specificity, and Clinical Application.
Vindhya K, et al.
Pharmacological Reviews, 64(3), 520-539 (2012)
The effects of antiviral treatment on breast cancer cell line.
Shaimerdenova M, et al.
Infectious Agents and Cancer, 12(1), 18-18 (2017)
Xiaoman Li et al.
Experimental hematology & oncology, 11(1), 2-2 (2022-01-22)
Tanshinone II A is an effective component extracted from Salvia miltiorrhiza and the roles of Tanshinone IIA in regulating the stemness of tumor cells remain unclear. This work aims to explore the roles and underlying mechanisms of Tanshinone IIA in
Ting Liu et al.
Bioengineered, 12(1), 7143-7155 (2021-09-22)
Cancer stem cell (CSC) has been confirmed to trigger tumor occurrence and progression and CSC can develop strategies to maintain a lower reactive oxygen species (ROS) level compared to cancer cells. However, the mechanisms contributing to ROS homeostasis in CSC
Xiaotian Xu et al.
Aging, 14(11), 4858-4873 (2022-06-10)
The roles of asparagine-linked glycosylation (ALG) members in tumorigenic process have been widely explored. However, their effects in colorectal cancer progression are still confusing. Here, we screened 12 ALGs' expression through online datasets and found that ALG10 was mostly upregulated


Cancer Stem Cell Detection Utilizing a Red-shifted Fluorescent Live Cell Probe for Aldehyde Dehydrogenase (ALDH), AldeRed™ 588-A.

AldeRed™ 588-A is a red fluorescent live cell probe that detects ALDH activity used to identify cancer stem cells and progenitor cells in culture. Cancer stem cells (CSCs) are subpopulations of cancer cells that can self-renew, generate diverse cells in the tumor mass, and sustain tumorigenesis. Some researchers believe that cancer arises from cancer stem cells that originate as a result of mutational hits on normal stem cells.

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