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MAK129

Sigma-Aldrich

β-Glucosidase Activity Assay Kit

sufficient for 100 colorimetric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

neurological disorders; gastrointestinal diseases; hematological disorder

storage temp.

−20°C

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MAK123G4171SAB1403853
vibrant-m

MAK129

β-Glucosidase Activity Assay Kit

vibrant-m

MAK123

α-Glucosidase Activity Assay Kit

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

usage

-

usage

-

detection method

colorimetric

detection method

colorimetric

detection method

-

detection method

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

neurological disorders; gastrointestinal diseases; hematological disorder

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders

relevant disease(s)

-

relevant disease(s)

-

General description

β-Glucosidase hydrolyzes carbohydrates by acting on terminal, non-reducing b(1→4)-linked D-glucose residues with the release of D-glucose. β-Glucosidases are required by organisms for the consumption of cellulose. Lysozyme, a β-glucosidase present in tears, acts on the β(1→4) glucose bonds present in the peptidoglycan cell wall of Gram-negative bacteria and helps to prevent bacterial infections in the eye. Defects in β-glucosidase activity have been implicated in Gaucher′s disease and Parkinson′s disease.

Application

β-Glucosidase Activity Assay Kit has been used to measure the β-glucosidase activity of the sample.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

The β-Glucosidase Activity Assay kit provides a simple and direct procedure for measuring β-glucosidase activity in biological samples, for characterizing β-glucosidase production, and for the screening of β-glucosidase modulators.

Principle

In this assay, β-glucosidase activity is determined by a reaction in which β-glucosidase hydrolyzes p-nitrophenyl-β-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the β-glucosidase activity present. One unit of β-Glucosidse is the amount of enzyme that catalyzes the hydrolysis of 1.0 μmole substrate per minute at pH 7.0.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2


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The approving agencies for plant protection agents request xenobiotic metabolism and residue studies in rats, farm animals and plants (e.g. EU regulation 1107/2009) according to OECD guidelines. The specific intestinal physiology of ruminants might lead to specific residues, which should
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