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MAK478

Sigma-Aldrich

α-Amylase Activity Assay Kit

new

sufficient for 100 colorimetric tests

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Quality Level

application(s)

agriculture
food and beverages

detection method

colorimetric

relevant disease(s)

endocrinological disorders; gastrointestinal diseases; genitourinary disorders

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Amylase belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4- glycosidic bonds. The α-amylases cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis, and renal failure.

Features and Benefits

  • The linear detection range of the kit is 0.3 to 50 U/L α-amylase.

Suitability

The kit is suitable for the determination of α-amylase activity in blood, saliva, urine, grains, and other agricultural samples.

Principle

Simple, direct, and automation-ready procedures for measuring amylase activity are very desirable. The α-Amylase Activity Assay Kit method involves two steps. First, α -amylase in the sample hydrolyses starch and the product is rapidly converted to glucose by α-glucosidase and hydrogen peroxide by glucose oxidase. Next, the hydrogen peroxide concentration is determined with a colorimetric reagent at 585 nm.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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