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11742027001

Roche

α-Glucosidase Assay

suitable for activity assay, kit of 1 (5 components)

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Synonym(s):
α-Glucosidase, Alpha-glucosidase activity assay kit, Maltase, glucosidase, glycosidase, neutral alpha-glucosidase (NAG)

usage

sufficient for 30 tests (and 10 blanks)

species reactivity

human

packaging

kit of 1 (5 components)

manufacturer/tradename

Roche

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

technique(s)

activity assay: suitable

detection method

colorimetric

storage temp.

2-8°C

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This Item
HPA026970SAB4500956NGLYF-RO
vibrant-m

11742027001

α-Glucosidase Assay

vibrant-m

NGLYF-RO

N-Glycosidase F

species reactivity

human

species reactivity

human

species reactivity

human, mouse

species reactivity

-

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

storage condition

-

storage condition

-

storage condition

-

usage

sufficient for 30 tests (and 10 blanks)

usage

-

usage

-

usage

-

technique(s)

activity assay: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:20-1:50

technique(s)

ELISA: 1:5000, western blot: 1:500-1:1000

technique(s)

-

General description

Colorimetric assay for the quantitative determination of α-glucosidase (maltase) in human semen research samples. α-Glucosidase is a key enzyme involved in carbohydrate hydrolysis. It is essential for the conversion of oligosaccharides and disaccharides into glucose.

application

α-Glucosidase Assay Kit has been used to measure neutral α-glucosidase (NAG) activity in seminal samples for the evaluation of epididymal function.

Features and Benefits

  • Fast and sensitive: The analysis can be performed using only 15 μl of semen or seminal plasma, ensuring accurate results while minimizing the sample volume needed.
  • Specific: The assay is designed to measure the activity of neutral α-glucosidase in human semen samples in life science research applications. It is not designed for diagnostic applications and cannot be used to measure α-glucosidase in other specimens.
  • Compatible: The assay is compatible with microplate assay formats and standard cuvette formats

Packaging

1 kit containing 5 components.

Components

The α-Glucosidase Assay contains the substrate and all buffers for the measurement of α-glucosidase in human semen or seminal plasma samples. It also contains a standard substance to set up a standard curve.

Preparation Note

Storage conditions (working solution): For stability of reconstituted kit components see section "General considerations".

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Reaction Buffer

  • Substrate Solution containing 4-nitrophenyl-α-D-glucopyranoside

  • Stopping Buffer

  • Castanospermine

  • Standard Solution containing 4-nitrophenol

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash


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Fatima Kazue Okada et al.
Reproduction & fertility, 1(1), 1-10 (2020-10-09)
According to the World Health Organization guidelines, ejaculatory abstinence (EA) of 2-7 days is recommended for semen analysis. This study aimed to determine how seminal quality may be affected by two EA periods from the same man. Seminal samples from
Emerging strategies for the activity assay and inhibitor screening of alpha-glucosidase
Zhang X, et al.
Food & Function, 11(1), 66-82 (2020)

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