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MAK276

Sigma-Aldrich

Sulfatase Activity Assay Kit (Colorimetric)

sufficient for 100 colorimetric tests

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NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

cancer

storage temp.

−20°C

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Lipase Activity Assay Kit sufficient for 100 colorimetric tests (with glycerol standard)

MAK046

Lipase Activity Assay Kit

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

cancer

relevant disease(s)

pulmonary disorders; gastrointestinal diseases; cancer

relevant disease(s)

cancer

relevant disease(s)

cardiovascular diseases; cancer; hematological disorder

General description

Sulfatases (EC 3.1.6) are a family of enzymes that catalyze the hydrolysis of sulfate ester bonds from a broad range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer.

Suitability

Suitable for measuring activity of purified enzyme as well as sulfatase from biological samples.

Principle

This Sulfatase Activity Assay Kit provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at 515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU.

pictograms

CorrosionHealth hazard

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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