MAK322

Collagen Assay Kit

sufficient for 100 fluorometric tests

• Synonym(s): Collagen Quantification Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 fluorometric tests
• input: cell; serum; plasma; solution(s) (collagen)
• application(s): cosmetics; food and beverages
• detection method: fluorometric
• relevant disease(s): rheumatological diseases; dermatological diseases; gastrointestinal diseases; pulmonary disorders; cancer
• storage temp.: −20°C

Description

General description

Collagen is the key structural protein of connective tissue and the most abundant protein in mammals. It occurs in many different types and forms with Types I -V being the most common. Aside from the crucial role it plays in the body, it has numerous medical applications such as its use in reconstructive surgery including bone and skin grafts. It is also commonly used in cosmetics due to its anti-aging and skin healing properties.

Application

The Collagen Assay Kit may be used for:

• Bone Disorder Research
• Dermatological Research
• Wound Healing and Tissue Repair Research

Features and Benefits

Enhanced Detection Range: Detect Collagen levels accurately across a wide range, from 2 µg/mL to 50 µg/mL, using only 20 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

Suitable for biological samples (e.g. plasma, serum, cells) and cosmetic samples (e.g. collagen solution)

Principle

Assay methods available for quantifying collagen currently range from needing extensive hydrolysis procedures with acids and bases to using expensive antibodies and complicated protocols. The collagen assay kit delivers a very simple, safe (non-radioactive), and sensitive method to quantify collagen in samples. In the first step of this procedure, collagen in the sample is enzymatically digested into peptides. Subsequently, the N-terminal glycine containing peptides react with the dye reagent to form a fluorescent complex. The fluorescence intensity of this product, measured at λex/em = 375/465 nm, is directly proportional to collagen concentration in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334,H360FD
• pcodes: P202 - P261 - P280 - P284 - P308 + P313 - P405
• Hazard Classifications: Repr. 1B - Resp. Sens. 1
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects