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Key Documents

MAK499

Sigma-Aldrich

Glutamate Dehydrogenase (GDH) Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Assay Kit for Glutamate Dehydrogenase Activity

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.84

Quality Level

application(s)

agriculture

detection method

colorimetric

relevant disease(s)

cancer; gastrointestinal diseases

shipped in

wet ice

storage temp.

−20°C

General description

Glutamate dehydrogenase (GLDH) is an enzyme which catalyzes the interconversion of glutamate and α-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the study of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections.

Features and Benefits

  • Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for glutamate dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue, and culture media.)

Principle

The non-radioactive, colorimetric Glutamate Dehydrogenase (GDH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2


Certificates of Analysis (COA)

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