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Key Documents

MBD0056

Sigma-Aldrich

Trace Elements Ready Made Solution

100X, microbial growth medium supplement

Synonym(s):

micronutrient

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.54

Quality Level

sterility

0.2 μm filtered

form

liquid

shelf life

2 yr

packaging

pkg of 50 mL

storage condition

protect from light

concentration

100 ×

suitability

suitable for (Media supplement)

shipped in

wet ice

storage temp.

2-8°C

General description

Minerals and vitamins are broadly used to supplement growth media in order to support growth of a variety of different bacteria. The growth media mimics the natural environment of the bacteria thus providing an optimal setting for high throughput cultures. Trace elements ready-made solution, MBD0056, is comprised of vital minerals to support critical bacterial development. The solution is based on Wolin and Wolfe’s recipe, which is especially suitable for culturing anaerobic bacteria of the human microbiome ( )

Features and Benefits

  • 0.2uM filtered for removal of contaminants
  • Saves time in preparation of media
  • Improved accuracy: composition and concentrations are analyzed and established by inductively coupled plasma (ICP)
  • Ready-made liquid mixture containing all ingredients.
  • Decreases exposure to chemical and toxic risks associated with the powder form of the trace elements

Components

50 mL Trace elements solution:
  • EDTA : 570 mg/L
  • CuSO4. 5H2O :10 mg/L
  • AlK(SO4)2 · 12H2O : 20 mg/L
  • H3BO3 :10 mg/L
  • Na2MoO4. 2H2O : 10 mg/L
  • Na2SeO3 : 1 mg/L
  • Na2WO4 . 2H2O : 10 mg/L
  • NiCl2 . 6H2O : 20 mg/L
  • MgSO4 . 7H2O : 3000 mg/L
  • MnSO4 . H2O : 500 mg/L
  • NaCl : 1000 mg/L
  • NH4Fe(SO4)2 · 12H2O :170 mg/L
  • Co(NO3)2 . 6H2O : 100 mg/L
  • CaCl2 · 2H2O :100 mg/L
  • ZnSO4 . 7H2O :100 mg/L

Other Notes

Add to pre-sterilized and cooled growth media. Concentration 100x.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Viologen dye inhibition of methane formation by Methanobacillus omelianskii
Wolin, E. A., R. S. Wolfe, and M. J. Wolin
Journal of Bacteriology, 87(5), 993-998 (1964)
Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice
Goodman, Andrew L., et al.
Proceedings of the National Academy of Sciences of the USA, 108(15), 6252-6257 (2011)
Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria
Ito, Tamaki, et al.
Gut Microbes, 10.1, 77-91 (2019)
Methanogens: reevaluation of a unique biological group
Balch, W. E., et al.
Microbiological Reviews, 43(2), 260-296 (1979)

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