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M9956

Sigma-Aldrich

M9 Minimal Salts, 5X

Liquid microbial growth medium

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Synonym(s):
5x M9 salts
NACRES:
NA.85

grade

for molecular biology

sterility

sterile; 0.2 μm filtered

form

liquid

suitability

suitable for nonselective for Escherichia coli and Coliforms

storage temp.

room temp

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L3272L3397M0643
Sigma-Aldrich

Sigma-Aldrich

M9956

M9 Minimal Salts, 5X

form

liquid

form

powder

form

powder

form

powder

sterility

sterile; 0.2 μm filtered

sterility

non-sterile

sterility

non-sterile

sterility

-

suitability

suitable for nonselective for Escherichia coli and Coliforms

suitability

-

suitability

suitable for molecular biology, nonselective for Escherichia coli, nonselective for coliforms

suitability

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

2-8°C

General description

M9 minimal media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.

Application

M9 Minimal Salts, 5X is suitable for non-selective cultivation of Escherichia coli strains for cloning, DNA plasmid production and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

M9 minimal media provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

15 g/L KH2PO4
64 g/L Na2HPO4 ⋅7H2O
2.5 g/L NaCl
5.0 g/L NH4Cl

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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MMYOMAG-74K-13

1000309185

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Protocols

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