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N3262

Sigma-Aldrich

NCTC 135 Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.13573 g/L
glucose: 1.0 g/L
phenol red: 0.02 g/L

shipped in

ambient

storage temp.

2-8°C

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NCTC 135 Medium

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technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.13573 g/L
glucose: 1.0 g/L
phenol red: 0.02 g/L

components

glucose: 1.0 g/L
phenol red: 0.02 g/L
L-glutamine: no
NaHCO3: 2.2 g/L

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

classic media, classical media, FBS, Mouse L-cell culture

mouse L929 cell cultue medium, carcinoma cell line establishment

keratinocye cloning medium, keratinocye long-term culture

HMVEC cloning medium, muscle cell culture medium

General description

NCTC 135 Medium is ideal for serum-free growth of mouse L-cells.

Application

NCTC 135 Medium has been used:
  • to culture Philophthalmus sp. rediae in vitro
  • to culture Ehrlich ascites mouse tumor cell line
  • as a culture media for the differentiation of Rcho-1 cells

Quantity

Formulated to contain 9.3 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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The American journal of pathology, 188(11), 2474-2486 (2018-08-31)
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The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
Synthesis, characterization and antitumor activity of 1, 2-disubstituted ferrocenes and cyclodextrin inclusion complexes
Petrovski Z, et al.
Journal of Organometallic Chemistry, 693, 675-684 (2008)
Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
Aiko Aoki et al.
The American journal of pathology, 188(11), 2474-2486 (2018-08-31)
Hypertensive disorder of pregnancy (HDP) is a serious pregnancy complication that is life threatening to both the mother and fetus. Understanding HDP pathophysiology is important for developing medical treatments. This study demonstrates the involvement of autophagy deficiency in adverse maternal

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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