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N7285

Sigma-Aldrich

Ninhydrin Reagent

2% Solution

Synonym(s):
1,2,3-Indantrione monohydrate, 2,2-dihydroxyindene-1,3-dione, Ninhydrine
EC Number:
NACRES:
NA.56

Quality Level

form

liquid

storage temp.

2-8°C

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This Item
72497N12861.06705
Sigma-Aldrich

Sigma-Aldrich

N7285

Ninhydrin Reagent

Sigma-Aldrich

Sigma-Aldrich

72497

Ninhydrin Reagent Set

Ninhydrin spray solution for thin-layer chromatography

Supelco

1.06705

Ninhydrin spray solution

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

15-25°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

Application

Ninhydrin reagent has been used to confirm the presence of the amino acid moieties in various reactions.
Ninhydrin reagents can be used in thin layer chromatography (TLC). Ninhydrin reagents have been used to develop and validate a simple, selective, and precise densitometric method for analysis of α-aminocephalosporins, both in bulk drugs and in formulations. Ninhydrin reagents have also been used to observe differences in the reference ranges of several plasma and urine amino acids between Singaporean and Caucasian populations.

Biochem/physiol Actions

Ninhydrin is a tricyclic 1,2,3-trione, which functions as an amino acid reagent. It is a vital organic building block, which exposes latent fingerprints on porous surfaces like paper, cardboard and raw wood. Ninhydrin is used to detect primary and secondary amines. It gives a strong blue color with primary amines and a less intense brown-red color with secondary amine.

Other Notes

Ninhydrin and hydrindantin in DMSO and lithium acetate buffer, pH 5.2

Caution

Store under nitrogen in the refrigerator after use

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Detection of lysyl oxidase-like 2 (LOXL2), a biomarker of metastasis from breast cancers using human blood samples
Janyasupab M, et al.
Recent patents on biomarkers, 5(2), 93-100 (2015)
Ninhydrin in synthesis of heterocyclic compounds.
Ziarani G, et al.
ARKIVOC (Gainesville, FL, United States), 2015 (2015)
Basic concepts of using solid phase synthesis to build small organic molecules using 2-chlorotrityl chloride resin
Bonkowski B, et al.
Modern Chemistry & Applications, 1(113), 2-2 (2014)
Catalytic reduction of pralidoxime in pharmaceuticals by macrocyclic Ni(II) compounds derived from orthophthalaldehyde
Reddy, P.M., et al.
Spectrochimica Acta Part A: Molecular Spectroscopy, 70, 704-712 (2008)
Low stocking density negatively affects growth, metabolism and stress pathways in juvenile specimens of meagre (Argyrosomus regius, Asso 1801)
Millan-Cubillo AF, et al.
Aquaculture (Amsterdam, Netherlands), 451, 87-92 (2016)

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