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O2260

Sigma-Aldrich

Osteopontin from mouse

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

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Synonym(s):
Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
MDL number:
NACRES:
NA.32

biological source

mouse

Quality Level

recombinant

expressed in NSO cells

sterility

sterile

assay

>95% (SDS-PAGE)

form

lyophilized powder

mol wt

30 kDa by SDS-PAGE (reducing)
65 kDa by SDS-PAGE (reducing) (doublet)

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Spp1(20750)

Application

Osteopontin from mouse has been used:
  • to study its role in a mouse model of preterm brain injury
  • as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques
  • to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels

Biochem/physiol Actions

Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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