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O3514

Sigma-Aldrich

Bovine Osteopontin

from bovine milk, powder, suitable for cell culture

Synonym(s):

Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)

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50 μG
$660.00

$660.00

Available to ship onApril 25, 2025Details

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50 μG
$660.00

About This Item

MDL number:
MFCD03100698
UNSPSC Code:
12352202
NACRES:
NA.32

$660.00

Available to ship onApril 25, 2025Details

Request a Bulk Order

Product Name

Osteopontin from bovine milk, lyophilized powder

biological source

bovine milk

Quality Level

100

sterility

sterile

form

lyophilized powder

mol wt

~60 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P31098
P31096

shipped in

ambient

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Osteopontin from bovine milk lyophilized powder

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Osteopontin from bovine milk

α-Lactalbumin from bovine milk Marker for non-denaturing PAGE

L4385

α-Lactalbumin from bovine milk

β-Lactoglobulin A from bovine milk ≥90% (PAGE)

L7880

β-Lactoglobulin A from bovine milk

Osteopontin human recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture

O4264

Osteopontin human

biological source

bovine milk

biological source

bovine milk

biological source

bovine milk

biological source

human

technique(s)

cell culture | mammalian: suitable

technique(s)

microbiological culture: suitable

technique(s)

HPLC: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile

sterility

-

sterility

-

sterility

sterile

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

mol wt

~60 kDa

mol wt

~14.2 kDa

mol wt

18,363 Da by calculation

mol wt

60-65 kDa by SDS-PAGE (reducing)

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

General description

Osteopontin (OPN) is a multifunctional protein. It is released by macrophages, T cells and epithelial cells.[1] It has 261 amino acid residues with a molecular mass of 29,283 Da.[2] Osteopontin is also called as uropontin.[3] Osteopontin from bovine milk is highly glycosylated and phosphorylated.[1] SPP1 gene (secreted phosphoprotein 1) codes for osteopontin. This gene is located on human chromosome 4 [3] and cow chromosome 6.[4] It has seven exons and six introns.[3]

Biochem/physiol Actions

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity.
Osteopontin stimulates cell mediated responses, chemotaxis of inflammatory cells and anti-inflammatory responses.[1] It binds to av-containing integrins and stimulate cell adhesion and migration.[3] Osteopontin promotes Th1 type immunity.[1]

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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    Certificates of Analysis (COA)

    Lot/Batch Number
    MKCW7112
    MKCV2704
    MKCS9932
    MKCN7062
    MKCM0986

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    Guidebook to the Extracellular Matrix, Anchor, and Adhesion Proteins (1999)
    Milk Proteins: From Expression to Food (2014)
    Milk and Milk Products in Human Nutrition (2011)
    Molecular analysis of the Bacillus subtilis bacteriophage SPP1 region encompassing genes 1 to 6: The products of gene 1 and gene 2 are required for pac cleavage.
    Chai S, et al.
    Journal of Molecular Biology, 224(1), 87-102 (1992)
    D D Hu et al.
    The Journal of biological chemistry, 270(44), 26232-26238 (1995-11-03)
    Osteopontin (OPN) is an extracellular matrix protein that binds to integrin alpha v beta 3. Here we demonstrate that two other integrins, alpha v beta 1 and alpha v beta 5, are also receptors for OPN. Human embryonic kidney 293
    View All Related Papers

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