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O3514

Sigma-Aldrich

Osteopontin from bovine milk

lyophilized powder

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Synonym(s):
Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
MDL number:
NACRES:
NA.32

biological source

bovine milk

Quality Level

sterility

sterile

form

lyophilized powder

mol wt

~60 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

Gene Information

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This Item
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Sigma-Aldrich

O3514

Osteopontin from bovine milk

α-Lactalbumin from bovine milk Type I, ≥85% (PAGE), lyophilized powder

L5385

α-Lactalbumin from bovine milk

Casein from bovine milk BioReagent, suitable for insect cell culture

C5679

Casein from bovine milk

α-Lactalbumin from bovine milk Type III, calcium depleted, ≥85% (PAGE), lyophilized powder

L6010

α-Lactalbumin from bovine milk

sterility

sterile

sterility

-

sterility

-

sterility

-

biological source

bovine milk

biological source

bovine milk

biological source

bovine milk

biological source

bovine milk

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

300

mol wt

~60 kDa

mol wt

-

mol wt

-

mol wt

14,178 Da by calculation

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

General description

Osteopontin (OPN) is a multifunctional protein. It is released by macrophages, T cells and epithelial cells. It has 261 amino acid residues with a molecular mass of 29,283 Da. Osteopontin is also called as uropontin. Osteopontin from bovine milk is highly glycosylated and phosphorylated. SPP1 gene (secreted phosphoprotein 1) codes for osteopontin. This gene is located on human chromosome 4 and cow chromosome 6. It has seven exons and six introns.

Biochem/physiol Actions

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity.
Osteopontin stimulates cell mediated responses, chemotaxis of inflammatory cells and anti-inflammatory responses. It binds to av-containing integrins and stimulate cell adhesion and migration. Osteopontin promotes Th1 type immunity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Milk and Milk Products in Human Nutrition (2011)
Milk Proteins: From Expression to Food (2014)
Molecular analysis of the Bacillus subtilis bacteriophage SPP1 region encompassing genes 1 to 6: The products of gene 1 and gene 2 are required for pac cleavage.
Chai S, et al.
Journal of Molecular Biology, 224(1), 87-102 (1992)
Guidebook to the Extracellular Matrix, Anchor, and Adhesion Proteins (1999)
D D Hu et al.
The Journal of biological chemistry, 270(44), 26232-26238 (1995-11-03)
Osteopontin (OPN) is an extracellular matrix protein that binds to integrin alpha v beta 3. Here we demonstrate that two other integrins, alpha v beta 1 and alpha v beta 5, are also receptors for OPN. Human embryonic kidney 293

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