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P0999

Sigma-Aldrich

Anti-Potassium Channel Kv10.1 (EAG-1) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

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Synonym(s):
Anti-Ether-a-go-go K+ channel 1, Anti-KCNH1
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human, mouse, rat

technique(s)

western blot: 1:200 using rat brain lysate and transfected cells HEK-KV10.1.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KCNH1(3756)
mouse ... Kcnh1(16510)
rat ... Kcnh1(65198)

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This Item
K0765P9497HPA018039
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

technique(s)

western blot: 1:200 using rat brain lysate and transfected cells HEK-KV10.1.

technique(s)

immunohistochemistry: suitable, western blot: 1:200 using rat brain and rat cortex lysates

technique(s)

western blot: 1:400 using the lysate of HEK 293 cells, stably expressing HERG

technique(s)

immunohistochemistry: 1:1000- 1:2500

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

Human genome codes for 40 voltage-gated K+ channels (KV). Voltage-gated-like ion channels (VGICs) are the third largest group of signaling molecules found in humans. K+ channels can be divided into four structural types based on their number of transmembrane regions and mode of activation, namely Kir- the inwardly rectifying 2-transmembrane K+ channels, K2P- 2-pore 4-transmembrane K+ channels, KCa- Ca2+-activated 6-transmembrane or 7-transmembrane K+ channels, and KV- voltage-gated 6-transmembrane K+ channels. KCNH1 (potassium voltage-gated channel H1) is a KV channel and is also known as KV10.1. Like all KV members, it is composed of four α-subunits with each containing six transmembrane α-helical segments, and a membrane-re-entering P-loop. These surround a central pore as homotetramers or heterotetramers. KCNH1 mRNA and protein are both abundantly found in the brain, though its peripheral tissue expression is restricted. This gene is localized to human chromosome 1q32.1.

Specificity

Specific for KV10.1 and does not cross react with KV10.2.

Immunogen

peptide GDPAKRKGWARFKDAC corresponding to residues 802-817 of rat KV10.1. This sequence is identical in mouse and has 14/16 residues identical in human.

Biochem/physiol Actions

KCNH1 (potassium voltage-gated channel H1) initiates a slowly activating, non-inactivating K+ current in a heterologous system. In skeletal muscles, it is expressed only during the phase when myoblasts exit the cell cycle and fuse, and thus, it plays an essential role in skeletal muscle development. This protein is found to be expressed in ∼70% human cancers, including gastric, mammary tumors, colon carcinoma, and sarcomas. This channel is implicated in neuronal disorders such as Zimmermann-Laband and Temple-Baraitser syndromes.

Target description

Potassium Channel Kv10.1 (EAG-1) encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming α-subunit of a voltage-gated non-inactivating delayed rectifier potassium channel.

Physical form

Lyophilized from phosphate buffered saline containing 1% bovine serum albumin and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Heike Wulff et al.
Nature reviews. Drug discovery, 8(12), 982-1001 (2009-12-02)
The human genome encodes 40 voltage-gated K(+) channels (K(V)), which are involved in diverse physiological processes ranging from repolarization of neuronal and cardiac action potentials, to regulating Ca(2+) signalling and cell volume, to driving cellular proliferation and migration. K(V) channels
Voltage-gated potassium channels: from hyperexcitability to excitement.
Pongs O
Febs Letters, 452(1-2), 31-35 (1999)
Bo Han et al.
Scientific reports, 6, 23417-23417 (2016-03-24)
Voltage-gated ether à go-go (EAG) K(+) channels are expressed in various types of cancer cells and also in the central nervous system. Aberrant overactivation of human EAG1 (hEAG1) channels is associated with cancer and neuronal disorders such as Zimmermann-Laband and

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