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Protease from Bacillus licheniformis

≥2.4 U/g

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Alcalase® 2.4L, Proteinase from Bacillus licheniformis, Subtilisin A
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:


aqueous solution

Quality Level

specific activity

≥2.4 U/g

storage temp.


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This Item
Proteinase, bacterial Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder



Proteinase, bacterial

specific activity

≥2.4 U/g

specific activity

7-15 units/mg solid

specific activity

≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid

specific activity

≥7 units/mg protein (biuret)

storage temp.


storage temp.


storage temp.


storage temp.


Quality Level


Quality Level


Quality Level


Quality Level


General description

Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.


Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations.
The enzyme from Sigma has been used to prepare chicken (leg) bone protein hydrolysates while evaluating ACE inhibitory peptides. It has also been used for the hydrolysis of Chlorella pyrenoidosa protein extracts during a study of their antitumor activities.

Biochem/physiol Actions

Alcalase is an endo-protease of the serine type. It has a broad substrate specificity and can hydrolyze most peptide bonds within a protein molecule. It is active between pH 6.5 and 8.5 and has an optimum temperature of 60 °C. The enzyme is is used in detergent formulations to remove protein-based stains.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Analysis Note

Activity expressed in Anson Units

Legal Information

A product of Novozyme Corp.
Alcalase is a registered trademark of Novozymes Corp.

Signal Word


Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids



Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion
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Neurolysin (Nln) is a recently recognized endogenous mechanism functioning to preserve the brain from ischemic injury. To further understand the pathophysiological function of this peptidase in stroke and other neurologic disorders, the present study was designed to identify small molecule
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Fish protein hydrolysates (FPH) obtained from industrial processing residues are sources of bioactive peptides. The enzymatic hydrolysis process is essential in obtaining specific bioactivities such as inhibition of the enzyme acetylcholinesterase (AChE). In this study the effect of different hydrolysis

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