Skip to Content
MilliporeSigma
All Photos(1)

Documents

P3910

Sigma-Aldrich

Protease from Bacillus licheniformis

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

Synonym(s):

Proteinase from Bacillus licheniformis, Subtilisin A

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9014-01-1
Enzyme Commission number:
3.4.21.62 (BRENDA, IUBMB)
EC Number:
232-752-2
MDL number:
MFCD00132092
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.84

form

lyophilized powder

quality

for use in Total Dietary Fiber Assay, TDF-100A

mol wt

27 kDa

application(s)

food and beverages

shipped in

wet ice

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Application

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

John Szpylka et al.
Journal of AOAC International, 95(6), 1547-1554 (2013-03-05)
The method for "Determination of Total Folates in Infant Formula and Adult Nutritionals by Trienzyme Extraction and UPLC-MS/MS Quantitation" was submitted to the Folate Working Group for consideration for adoption as Official First Action by AOAC INTERNATIONAL. This method uses
Micaela Vannini et al.
Polymers, 13(7) (2021-04-04)
With the aim to fully exploit the by-products obtained after the industrial extraction of starch from sweet potatoes, a cascading approach was developed to extract high-value molecules, such as proteins and pectins, and to valorize the solid fraction, rich in
Katja Kantanen et al.
Foods (Basel, Switzerland), 11(9) (2022-05-15)
Faba bean is a potential ingredient due to its high protein yield and its possible cultivation in colder climate regions. In this study, meat analogues made from faba bean protein isolate (FPI) and concentrate (FPC) blends were produced using high
Zhaoyue Men et al.
Journal of animal science and biotechnology, 13(1), 14-14 (2022-01-17)
Dietary fiber (DF) is often eschewed in swine diet due to its anti-nutritional effects, but DF is attracting growing attention for its reproductive benefits. The objective of this study was to investigate the effects of DF intake level on oocyte
Yong Zhuo et al.
Journal of animal science and biotechnology, 11, 47-47 (2020-05-20)
This study aimed to investigate the impacts of guar gum and cellulose as the source of dietary fiber during gestation on the reproductive performance of sows. A total of 210 sows (parities 3-6) were randomly allocated into six diets (n = 35)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service