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P3910

Sigma-Aldrich

Protease from Bacillus licheniformis

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

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Synonym(s):
Proteinase from Bacillus licheniformis, Subtilisin A
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.84

form

lyophilized powder

Quality Level

quality

for use in Total Dietary Fiber Assay, TDF-100A

mol wt

27 kDa

application(s)

food and beverages

shipped in

wet ice

storage temp.

2-8°C

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This Item
P4860P5459P8038
Proteinase, bacterial Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

Sigma-Aldrich

P8038

Proteinase, bacterial

mol wt

27 kDa

mol wt

-

mol wt

-

mol wt

27 kDa

application(s)

food and beverages

application(s)

-

application(s)

-

application(s)

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Application

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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