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P5459

Sigma-Aldrich

Protease from Bacillus licheniformis

glycerol solution (50%)

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Synonym(s):
Proteinase from Bacillus licheniformis, Subtilisin A
CAS Number:
Enzyme Commission number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.54

biological source

Bacillus licheniformis

form

glycerol solution (50%)

specific activity

≥7 units/mg protein (biuret)

concentration

≥50 mg/mL

solubility

0.85% sodium chloride: soluble, clear (Diluted)
H2O: miscible

storage temp.

2-8°C

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This Item
P5380P8038P4860
Proteinase, bacterial Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

Sigma-Aldrich

P8038

Proteinase, bacterial

form

glycerol solution (50%)

form

lyophilized powder

form

lyophilized powder

form

aqueous solution

specific activity

≥7 units/mg protein (biuret)

specific activity

7-15 units/mg solid

specific activity

≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid

specific activity

≥2.4 U/g

concentration

≥50 mg/mL

concentration

-

concentration

-

concentration

-

solubility

0.85% sodium chloride: soluble, clear (Diluted), H2O: miscible

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear, H2O: soluble, colorless

solubility

-

solubility

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

General description

The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.

Application

Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Packaging

Package size based on protein content.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Pictograms

CorrosionHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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