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P3026

Sigma-Aldrich

Pectolyase from Aspergillus japonicus

greener alternative

lyophilized powder, ≥0.3 units/mg solid

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Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

Aspergillus sp. (A. japonicus)

Quality Level

form

lyophilized powder

specific activity

≥0.3 units/mg solid

mol wt

50 kDa

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative category

storage temp.

2-8°C

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This Item
P5936P2611A9857
form

lyophilized powder

form

lyophilized powder

form

aqueous solution

form

powder

specific activity

≥0.3 units/mg solid

specific activity

≥0.3 unit/mg solid

specific activity

≥3,800 units/mL

specific activity

≥150 units/mg protein (biuret)

mol wt

50 kDa

mol wt

-

mol wt

-

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.

Application

Pectolyase from Aspergillus japonicus has been used:
  • in the lysis of chlorella cells for protoplast generation
  • as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation
  • to induce reorganization of Arabidopsis root cap cell architecture

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis.
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Biochem/physiol Actions

Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora.
Pectolyase is used for protoplast isolation from plant cells. It also mediates ethylene hormone synthesis.

Other Notes

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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705578-5MG-PW

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Customers Also Viewed

Isolation and properties of pectinases from the fungus Aspergillus japonicus
Semenova MV, et al.
Biochemistry (Moscow), 68(5), 559-569 (2003)
Pectic enzymes in pectolyase: separation, characterization, and induction of ethylene in fruits
Baldwin EA and Pressey R
Plant Physiology, 90(1), 191-196 (1989)
P J De Lange et al.
Annals of botany, 101(6), 881-899 (2007-12-07)
Crassula hunua and C. ruamahanga have been taxonomically controversial. Here their distinctiveness is assessed so that their taxonomic and conservation status can be clarified. Populations of these two species were analysed using morphological, chromosomal and DNA sequence data. It proved
Susann Hesse et al.
Frontiers in plant science, 10, 773-773 (2019-07-10)
During prophase I a meiosis-specific proteinaceous tripartite structure, the synaptonemal complex (SC), forms a scaffold to connect homologous chromosomes along their lengths. This process, called synapsis, is required in most organisms to promote recombination between homologs facilitating genetic variability and
Identification of sporopollenin as the outer layer of cell wall in microalga Chlorella protothecoides
He X, et al.
Frontiers in Microbiology, 7, 1047-1047 (2016)

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