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C8274

Sigma-Aldrich

Cytohelicase from Helix pomatia

lyophilized powder

Synonym(s):

Snail gut enzyme

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

composition

Protein, 50-80% Lowry

storage temp.

2-8°C

Application

Cytohelicase from Helix pomatia has been used:
  • in the enzyme solution for preparing the cell nuclei for immuno fluorescent in situ hybridization (immunoFISH)
  • in the enzyme mix to analyze meiotic cells at diakinesis and later stages in anthers
  • in the enzyme mix for the analysis of mitosis in anthers

Cytohelicase from Helix pomatia has been used to study the isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia.
The enzyme from Sigma has been used for cytohelicase assay. The enzyme assay was used while studying the effects of MNN10 gene-disruption on protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae cultures.

Biochem/physiol Actions

Cytohelicase from Helix pomatia is used in the preparation of synaptonemal complex spreading techniques along with bovine serum albumin. It plays an important role in the digestion of cell wall. Cytohelicase interacts with chromatin and improves the adhesion of synaptonemal complexes to the hydrophobic plastic film.

Analysis Note

Contains glucanase/laminarinase activity

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ribosomal DNA locus evolution in Nemesia: transposition rather than structural rearrangement as the key mechanism?
Datson PM and Murray BG
Chromosome Research, 14(8), 845-857 (2006)
Mikhail G Divashuk et al.
PloS one, 9(1), e85118-e85118 (2014-01-28)
Hemp (Cannabis sativa L.) was karyotyped using by DAPI/C-banding staining to provide chromosome measurements, and by fluorescence in situ hybridization with probes for 45 rDNA (pTa71), 5S rDNA (pCT4.2), a subtelomeric repeat (CS-1) and the Arabidopsis telomere probes. The karyotype
Danguole Bartkeviciūte et al.
FEMS yeast research, 4(8), 833-840 (2004-09-29)
Screening for genes affecting super-secreting phenotype of the over-secreting mutant of Kluyveromyces lactis resulted in isolation of the gene named KlMNN10, sharing high homology with Saccharomyces cerevisiae MNN10. The disruption of the KlMNN10 in Kluyveromyces lactis, as well as of
ImmunoFISH: simultaneous visualisation of proteins and DNA sequences gives insight into meiotic processes in nuclei of grasses.
Sepsi A, et al.
Frontiers in Plant Science, 9(7), 1193-1193 (2018)
M Diatewa et al.
Biochimie, 63(1), 67-69 (1981-01-01)
Conversion of large amounts of Saccharomyces cerevisiae cells to protoplasts is studied using various preparations extracted from Helix pomatia hepato-pancreatic juice. The most favourable yield in two hours incubations (88 per cent) is obtained with 20 ml cytohelicase, a chitinase

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