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P7937

Sigma-Aldrich

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit

5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder

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Enzyme Commission number:
MDL number:
NACRES:
NA.32

biological source

rabbit

Quality Level

recombinant

expressed in E. coli

assay

≥80% (GE)

form

lyophilized powder

specific activity

5,000-15,000 units/mg protein

mol wt

37.5 kDa

technique(s)

inhibition assay: suitable

UniProt accession no.

storage temp.

−20°C

Gene Information

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This Item
P6993P6998C8482
Sigma-Aldrich

P6993

Protein Phosphatase 2A1 bovine

Gene Information

rabbit ... PPP1CA(100009298)

Gene Information

-

Gene Information

human ... PRKACB(5567)

Gene Information

human ... PRKACA(5566)

technique(s)

inhibition assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

assay

≥80% (GE)

assay

≥90% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

>90% (SDS-PAGE)

biological source

rabbit

biological source

bovine

biological source

-

biological source

-

form

lyophilized powder

form

solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

General description

Protein phosphatase1 (PP1) is a heterodimeric enzyme with serine/threonine phosphatase activity. It comprises a catalytic subunit and a targeting subunit or a specific protein inhibitor. The targeting subunit determines the enzyme localization, e.g., to glycogen particles (subunit G) or to myofibrils (subunit M).

Application

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used as a standard in protein phosphatase-1 inhibition assay.
Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used in protein phosphatase reaction and in the digestion of adipocyte extracts.

Biochem/physiol Actions

Protein phosphatase1 (PP1) is involved in glycogen metabolism and in regulation of muscle contractility. In addition, it is implicated in cell cycle and transcriptional regulation. Recombinant rabbit PP1α has properties in common with the native rabbit muscle protein including size, requirement for Mn2+ for activity, specificity for phosphorylase A, and inhibition by okadaic acid, microcystin LR, and phosphatase inhibitor2 (I-2).

Packaging

Package size based on protein content.

Unit Definition

One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.4 at 30 °C.

Physical form

Lyophilized powder containing imidazole buffer, pH 7.4, NaCl, DTT, EDTA, MnCl2, Tween 20, and trehalose as stabilizer.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Satomi Ohtsuka et al.
Biochemistry, 59(17), 1701-1710 (2020-04-17)
Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) activates particular multifunctional kinases, including CaMKI, CaMKIV, and 5'AMP-activated protein kinase (AMPK), resulting in the regulation of various Ca2+-dependent cellular processes, including neuronal, metabolic, and pathophysiological pathways. We developed and characterized a novel pan-CaMKK inhibitor
M C Faux et al.
Trends in biochemical sciences, 21(8), 312-315 (1996-08-01)
One important regulatory mechanism in the control of phosphorylation events is the subcellular location of phosphatases of kinases. Several serine/threonine phosphatases and kinases have now been shown to be associated with targeting subunits; these contribute to the organization and specificity
Insulin resistance of glycogen synthase mediated by o-linked N-acetylglucosamine.
Parker GJ
The Journal of Biological Chemistry, 278(12), 10022-10027 (2003)
A J Zhang et al.
The Journal of biological chemistry, 267(3), 1484-1490 (1992-01-25)
The catalytic subunit of rabbit skeletal muscle protein phosphatase-1 was expressed in Escherichia coli. Expression of phosphatase-1 in the pET3a vector, which is based on the use of the T7 promoter, resulted in the expression of the enzyme as an
Regulation of protein phosphatase-1
James B Aggen
Chemistry and Biology, 7.1, R13-R23 (2000)

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