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P7965

Sigma-Aldrich

Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse

clone F4, ascites fluid

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Synonym(s):
Anti-ABC20, Anti-CD243, Anti-CLCS, Anti-GP170, Anti-MDR1, Anti-P-GP, Anti-PGY1, Anti-p-170
MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

F4, monoclonal

mol wt

antigen 170-180 kDa

contains

15 mM sodium azide

species reactivity

hamster, human

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human kidney sections
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
radioimmunoassay: suitable using cell-surface RIA
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ABCB1(5243)

Related Categories

General description

The ABCB1 (ATP binding cassette subfamily B member 1) gene is mapped to human chromosome 7q21.12. It encodes for P-glycoprotein, which is a membrane bound drug transporter protein, belonging to the ATP-binding cassette (ABC) transporters family. The gene is considered to be highly polymorphic.

Specificity

The antibody recognizes an epitope located in the amino terminal half of P-glycoprotein (Pgp), at the third extracellular loop of the molecule. The epitope is resistant to formalin fixation and periodate oxidation. The antibody detects specifically human MDR1 P-glycoprotein, but does not appear to recognize the human MDR3 product, nor the mouse mdr1a, mdr1b or the mdr3 P-glycoprotein.

Immunogen

mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

Application

Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse has been used in western blot analysis and Immunohistochemistry.

Biochem/physiol Actions

Overexpression of ABCB1 (ATP binding cassette subfamily B member 1) gene is a major cause for multi-drug resistance, which makes chemotherapy challenging for the treatment of osteosarcoma. P-glycoprotein (P-gp) mediates the energy-dependent efflux of xenobiotic to the outside of plasma membrane from the inside of the cell , thereby affecting the process of absorption, distribution, and excretion of drugs. Cyclosporine, a calcineurin inhibitor serves as a substrate for P-gp.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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ABCB1 polymorphism predicts escitalopram dose needed for remission in major depression.
Singh A B, et al.
Translational Psychiatry, 2(11), e198-e198 (2012)
Targeting ABCB1 (MDR1) in multi-drug resistant osteosarcoma cells using the CRISPR-Cas9 system to reverse drug resistance.
Liu T, et al.
Oncotarget, 7(50), 83502-83502 (2016)
Yun-Kai Zhang et al.
Oncotarget, 6(27), 24277-24290 (2015-08-25)
Overexpression of ATP-Binding Cassette transporters leads to multidrug resistance in cancer cells and results in the failure of chemotherapy. In this in-vitro study, we investigated whether or not (20S, 24R/S)-epoxy-12β, 25-dihydroxy-dommarane-3β-amine (ORA and OSA), a pair of semi-synthetic ocotillol analogue
Effect of MDR1 C1236T polymorphism on cyclosporine pharmacokinetics: A systematic review and meta-analysis.
Chen Z, et al.
Medicine, 96(47) (2017)
Di-2-pyridylketone 4, 4-Dimethyl-3-thiosemicarbazone (Dp44mT) Overcomes Multidrug-Resistance by a Novel Mechanism Involving the Hijacking of Lysosomal P-Glycoprotein (Pgp).
Jansson P J, et al.
The Journal of Biological Chemistry (2015)

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