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PKH26GL

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

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Synonym(s):
Red PKH membrane dye
NACRES:
NA.32

packaging

pkg of 1 kit

Quality Level

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

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This Item
PKH26PCLMIDI26MIDI67
manufacturer/tradename

Distributed for Phanos Technologies

manufacturer/tradename

-

manufacturer/tradename

Distributed for Phanos Technologies

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

storage condition

-

storage condition

protect from light

storage condition

protect from light

fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

fluorescence

-

fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

detection method

fluorometric

detection method

-

detection method

fluorometric

detection method

fluorometric

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

ambient

General description

PKH26 is a red‐fluorescent dye. This lipophilic membrane dye is widely used to determine cell proliferation in heterogeneous cells by flow cytometry.

Application

PKH26 Red Fluorescent Cell Linker Kit is for general cell membrane labeling. It has been characterized in a number of model systems and has been found to be useful for in vitro cell labeling, in vitro proliferation studies and long term, in vivo cell tracking. The half-life for elution of PKH26 from labeled rabbit red blood cells is greater than 100 days. This enhanced stability is favorable for long term in vivo studies.
This kit is for general cell membrane labeling. It has been characterized in a number of model systems and has been found to be useful for in vitro cell labeling, in vitro proliferation studies and long term, in vivo cell tracking. The half-life for elution of PKH26 from labeled rabbit red blood cells is greater than 100 days. This enhanced stability is favorable for long term in vivo studies.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of Phanos Technologies

Kit Components Only

Product No.
Description

  • Diluent C 6 x 10

  • PKH26 Cell Linker in ethanol .5 mL

related product

pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

57.2 °F - closed cup

flash_point_c

14.0 °C - closed cup


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Anne Julie Frenette et al.
Journal of neurotrauma, 29(1), 1-18 (2011-08-19)
In the intensive care unit, dopamine agonists (DA) have been used in traumatic brain injury (TBI) patients to augment or accelerate cognitive recovery and rehabilitation. However, the efficacy and safety of DA in this population is not well established. We
Ofra Ben Menachem-Zidon et al.
PloS one, 14(6), e0218081-e0218081 (2019-06-14)
The beneficial effect of mesenchymal stem cells (MSCs) on wound healing is mostly attributed to a trophic effect that promotes angiogenesis. Whether MSCs can contribute to the formation of new blood vessels by direct differentiation is still controversial. Pelvic floor
Tracking antigen-driven responses by flow cytometry: Monitoring proliferation by dye dilution
Paul KW, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 73(11), 1019-1034 (2008)
Rafi Chapanian et al.
Biomaterials, 33(10), 3047-3057 (2012-01-21)
The in vivo circulation of hyperbranched polyglycerol (HPG) grafted red blood cells (RBCs) was investigated in mice. The number of HPG molecules grafted per RBC was measured using tritium labeled HPGs ((3)H-HPG) of different molecular weights; the values ranged from
EphA3 as a target for antibody immunotherapy in acute lymphoblastic leukemia
Charmsaz S, et al.
Leukemia, 31(8), 1779-1779 (2017)

Articles

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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