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ProteoPrep® Universal Extraction Kit

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This kit features new and innovative detergents, and uses specially formulated reagents and an optimized protocol designed to generate two prepared subcellular fractions that are uniquely ready for two-dimensional (2D) electrophoresis.
  • Fraction 1: Soluble/Cytoplasmic Proteins
  • Fraction 2: Membrane Proteins
The special reducing and alkylating reagents produce samples that exhibit improved focusing and decreased streaking in 2D gels. The ProteoPrep Universal Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Features and Benefits

  • Innovative detergent preparations - Highly improved solubility allows for higher protein loads and greater visibility of low abundance proteins on 2D gels.
  • Two pre-mixed solubilizaton solutions - Generates two distinct populations for easy 2D analysis.
  • Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
  • Pre-weighed dry blends - Stable and easy to reconstitute
  • Conveniently Packaged - No waste. Use only the amount needed.

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.

  • C0356Protein Extraction Reagent Type 4 23 mLSDS

  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5SDS

  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56SDS



Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3


Respiratory system

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects




Not applicable


Not applicable

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