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R4142

Sigma-Aldrich

RNA Marker template set

~0.5 mg/mL in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA

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About This Item

UNSPSC Code:
12352200

grade

for molecular biology

concentration

~0.5 mg/mL in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA

storage temp.

−20°C

Application

In vitro transcription with T7 RNA polymerase produces transcripts 100, 200, 300, 400, 600, 800 and 1,000 bases long. The transcripts can be labeled by including biotinylated, radioactive, or other labeled rNTP′s in the reaction. The markers are useful for RNase protection assays or for electrophoresis of low molecular weight RNA fragments.

Other Notes

A mixture of seven linearized plasmids for use as templates for RNA markers.

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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