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R8000

Sigma-Aldrich

D-Ribulose 1,5-Diphosphate Carboxylase from spinach

partially purified powder, 0.01-0.1 unit/mg solid

Synonym(s):

3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

spinach

Quality Level

form

partially purified powder

specific activity

0.01-0.1 unit/mg solid

mol wt

557 kDa

storage temp.

−20°C

General description

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.[1]
Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

Application

D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:
  • as a test protein in pepsin digestion studies[2]
  • as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines[3]
  • in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid[4]

Biochem/physiol Actions

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation.[5] It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate.[1] Abscisic acid inhibits the carboxylation activity of Rubisco.[4]

Unit Definition

One unit will convert 1.0 μmole of D-RuDP and CO2 to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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    Libor Vítek et al.
    Antioxidants (Basel, Switzerland), 11(11) (2022-11-12)
    Oxidative stress and inflammation contribute significantly to atherogenesis. We and others have demonstrated that mildly elevated serum bilirubin levels protect against coronary and peripheral atherosclerosis, most likely due to the antioxidant and anti-inflammatory activities of bilirubin. The aim of the
    Hiroyuki Ishida et al.
    Biochimica et biophysica acta, 1837(4), 512-521 (2013-11-26)
    Chloroplasts are the primary energy suppliers for plants, and much of the total leaf nitrogen is distributed to these organelles. During growth and reproduction, chloroplasts in turn represent a major source of nitrogen to be recovered from senescing leaves and
    Maya Kremien et al.
    Proceedings of the National Academy of Sciences of the United States of America, 110(22), 8978-8983 (2013-04-24)
    Soft corals of the family Xeniidae exhibit a unique, rhythmic pulsation of their tentacles (Movie S1), first noted by Lamarck nearly 200 y ago. However, the adaptive benefit of this perpetual, energetically costly motion is poorly understood. Using in situ
    Yuji Suzuki et al.
    Journal of experimental botany, 64(4), 1145-1152 (2013-01-26)
    Rubisco gene expression was examined in detail in rice (Oryza sativa L.) leaves at different positions, i.e. expanding, mature, and senescent leaves. Rubisco small subunit (RBCS) synthesis and RBCS mRNA levels were maximal in expanding leaves and gradually became lower
    Michael J Bayly et al.
    Molecular phylogenetics and evolution, 69(3), 704-716 (2013-07-24)
    We present a phylogenetic analysis and comparison of structural features of chloroplast genomes for 39 species of the eucalypt group (genera Eucalyptus, Corymbia, Angophora, and outgroups Allosyncarpia and Stockwellia). We use 41 complete chloroplast genome sequences, adding 39 finished-quality chloroplast

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