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R8005

SAFC

RPMI-1640 Medium

Hybri-Max, Modified, with L-glutamine, 4500 mg/L glucose and 15mM HEPES, without sodium bicarbonate, powder, suitable for hybridoma

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MDL number:
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
NaHCO3: no
glucose: 4.5 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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1 of 4

This Item
R413051536CR1780
vibrant-m

R8005

RPMI-1640 Medium

vibrant-m

R4130

RPMI-1640 Medium

vibrant-m

51536C

RPMI-1640 Medium

vibrant-m

R1780

RPMI-1640 Medium

form

powder

form

powder

form

liquid

form

liquid

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
NaHCO3: no
glucose: 4.5 g/L (Dextro)

components

L-glutamine: yes
NaHCO3: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no

components

HEPES: 5958 mg/L
L-glutamine: 300 mg/L
NaHCO3: 2000 mg/L
phenol red: 5.310 mg/L

components

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

impurities

endotoxin

impurities

-

impurities

-

impurities

endotoxin, tested

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Quantity

Formulated to contain 16.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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