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RAB0380

Sigma-Aldrich

Human β-NGF ELISA Kit

for serum, plasma, cell culture supernatant and urine

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Synonym(s):
Beta-NGF, Beta-nerve growth factor 
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NGF(4803)

General description

The Human β-NGF (β-nerve growth factor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human β-NGF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human BNGF

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human β-NGF (nerve growth factor) ELISA (Enzyme linked immunosorbent assay) Kit has been used for the quantification of nerve growth factor in acellularized meningeal scaffolds (AMS) and recovered meningeal tissues (referred as CMS) , in all collected medium culture wastes. It has also been used for the quantification of nerve growth factor in lysate of decellularized meningeal scaffolds (DMS).

Biochem/physiol Actions

NGFβ (nerve growth factor β ) is a potent neurotrophic factor that plays a vital role in development and maintenance of nervous systems and is essential for the differentiation and survival of neurons. It also mediates angiogenesis and facilitates progression and development of various cancer including, oral, breast and prostate cancers. Elevated expression of NGFβ contributes to progression of human cholangiocarcinoma. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB) and might also aid in determining the therapy for the same. It might alter placental function, as NGF signaling in placenta is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8B - Non-combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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25G
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Prospect of umbilical cord mesenchymal stem cell culture waste in regenerative medicine
Pawitan J, et al.
Journal of Global Pharma Technology (2009)
A membrane form of guanylate cyclase is an atrial natriuretic peptide receptor.
Chinkers M
Nature, 338(6210), 78-83 (1989)
Sandeep Kumar Vishwakarma et al.
Materials science & engineering. C, Materials for biological applications, 102, 34-44 (2019-05-31)
Spinal cord injury (SCI) is one of the most precarious conditions which have been one of the major reasons for continuous increasing mortality rate of SCI patients. Currently, there is no effective treatment modality for SCI patients posing major threat
Hong-Chen Qu et al.
PloS one, 9(9), e106321-e106321 (2014-09-03)
To examine whether urinary nerve growth factor (NGF) could serve as a biomarker for interstitial cystitis/painful bladder syndrome (IC/PBS), we conducted a comprehensive meta-analysis of 9 studies. Among the studies considered, patients with IC/PBS had higher urinary NGF and NGF/Cr
Xiaoyin Ke et al.
Drug and alcohol dependence, 142, 290-294 (2014-07-30)
This study investigated the serum levels of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in a group of chronic ketamine abusers in comparison to healthy controls. The correlations between the serum BDNF, NGF level with the subjects' demographic

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