Skip to Content
MilliporeSigma
All Photos(2)

Documents

RAB0472

Sigma-Aldrich

Human TIMP-2 ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

Metalloproteinase inhibitor 2 , TIMP-2, Tissue inhibitor of metalloproteinases 2

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 1.6-1200 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TIMP2(7077)

General description

The Human TIMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TIMP-2 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human TIMP2

Application

For research use only. Not for use in diagnostic procedures.
Human TIMP-2 ELISA Kit has been used to measure tissue inhibitor of metalloproteinase-2 (TIMP-2) level in blood samples from Atherosclerotic renal artery stenosis (ARAS) patients.

Biochem/physiol Actions

Tissue inhibitor of metalloproteinase-2 (TIMP-2) plays a vital role in inhibition of matrix metalloproteinases (MMPs) and functions as a key modulator of extracellular matrix deposition and degradation in various physiological processes. TIMP-2 can also act through a matrix metalloproteinase (MMP)-independent mechanism inhibiting endothelial cell proliferation in vitro. TIMP-2 mediates anti-angiogenic activities in vivo. TIMP-2 is a target gene of the microRNA-miR-22. TIMP-2 functions as a biomarker for cell cycle arrest.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

TIMP-2 mediated inhibition of angiogenesis: an MMP-independent mechanism.
Seo DW
Cell, 114(2), 171-180 (2003)
A Eirin et al.
Acta physiologica (Oxford, England), 226(3), e13267-e13267 (2019-02-26)
Patients with renovascular hypertension (RVH) exhibit elevated urinary mtDNA copy numbers, considered to constitute surrogate markers of renal mitochondrial injury. The modest success of percutaneous transluminal renal angioplasty (PTRA) in restoring renal function in RVH has been postulated to be
Changes in inflammatory biomarkers after renal revascularization in atherosclerotic renal artery stenosis.
Wang W
Nephrology, Dialysis, and Transplantation, 31(9), 1437-1443 (2016)
Tissue inhibitors of metalloproteinases: structure, regulation and biological functions.
Gomez DE
European Journal of Cell Biology, 74(2), 111-122 (1997)
Atherosclerotic renal artery stenosis is associated with elevated cell cycle arrest markers related to reduced renal blood flow and postcontrast hypoxia.
Saad A
Nephrology, Dialysis, and Transplantation, 31(11), 1855-1863 (2016)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service