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RAB0509

Sigma-Aldrich

Mouse VEGF ELISA Kit

for serum, plasma, cell culture supernatant

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Synonym(s):
VEGF-A, Vascular endothelial growth factor A 

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 4.1-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Vegfa(22339)

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Mouse VEGF ELISA Kit for serum, plasma, cell culture supernatant

RAB0509

Mouse VEGF ELISA Kit

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Mouse aFGF ELISA Kit

Rat VEGF ELISA Kit for serum, plasma and cell culture supernatant

RAB0511

Rat VEGF ELISA Kit

Human VEGF ELISA Kit for cell and tissue lysates

RAB0508

Human VEGF ELISA Kit

Gene Information

mouse ... Vegfa(22339)

Gene Information

mouse ... Fgf1(14164)

Gene Information

rat ... Vegfa(83785)

Gene Information

human ... VEGFA(7422)

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum sample(s)

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 4.1-1000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/ml

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 0.82-200 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Vascular endothelial growth factor (VEGF) is produced by thymus epithelial cells in adults. It is located on human chromosome 6p21.1 The Mouse VEGF ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VEGF in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Mouse VEGF-A

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse VEGF ELISA Kit has been used in biochemical analysis.

Biochem/physiol Actions

Vascular endothelial growth factor (VEGF) supports vascular homeostasis. Vascular endothelial growth factor-A (VEGF-A) modulates angiogenesis, vascular permeability and growth. It plays a vital role in the progression of the vasculature. Deficiency of VEGF-A leads to endothelial dysfunction, hypertension, ischemia and blood clotting.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Singla R, et al.
Carbohydrate Polymers, 155(1), 152-162 (2017)
Jia-Wen Zhan et al.
PloS one, 15(6), e0234747-e0234747 (2020-06-26)
The vascular buds in the vertebral endplate (VEP) are the structural foundation of nutrient exchange in the intervertebral disc (IVD). VEGF is closely related to angiogenesis in the endplate and intervertebral disc degeneration (IDD). To investigate the effects of static
Doxycycline modulates VEGF-A expression: Failure of doxycycline-inducible lentivirus shRNA vector to knockdown VEGF-A expression in transgenic mice
Merentie M, et al.
Testing, 13(1), e0190981-e0190981 (2018)
REGULATION OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) PRODUCTION BY MOUSE THYMIC EPITHELIAL CELL LINES.
Rutto K V, et al.
Tsitologiia, 58(6), 436-443 (2016)
Rubbel Singla et al.
Scientific reports, 7(1), 10457-10457 (2017-09-07)
The ever increasing incidences of non-healing skin wounds have paved way for many efforts on the convoluted process of wound healing. Unfortunately, the lack of relevance and success of modern wound dressings in healing of acute and diabetic wounds still

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