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RAB0460

Sigma-Aldrich

Human TGF-β 1 ELISA Kit

for serum, plasma, and cell culture supernatants

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Synonym(s):
TGF-beta-1, Transforming growth factor beta-1
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.0823-60 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TGFB1(7040)

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RAB0461RAB0738RAB0458
packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type urine
sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.0823-60 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 40 pg/mL
standard curve range: 40.96-10000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 220 pg/mL
standard curve range: 0.205-50 ng/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Human TGF-β 1 ELISA (Enzyme-Linked mmunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TGF-β 1 in serum, plasma, cell culture supernatants.

Immunogen

Recombinant Human TGF-β 1

Application

For research use only. Not for use in diagnostic procedures.
Human transforming growth factor-β1 (TGF-β1) ELISA Kit has been used to measure level of TGF-β1 released from tubular cells.

Biochem/physiol Actions

Transforming growth factor-β1 (TGF-β1) is a pluripotential cytokine that plays a vital role in the homeostasis of peripheral CD4+CD25+ regulatory T cells and regulation of FOXP3 (forkhead box P3) expression in vivo. Binding of TGF-β1 to its receptor, activates members of the SMAD protein family involved in gene regulation. Study shows that reduced expression of circulating transforming growth factor-β1 (TGF-β1) might contribute to systemic lupus erythematosus (SLE) development. Polymorphism of TGF-β1 is associated with pathogenesis of rheumatoid arthritis (RA) and pneumoconiosis. Serum TGF-β1 expression is elevated in systemic sclerosis (SSc) patients. Serum TGF-β1 functions as a potential biomarker of Guillain-Barré syndrome (GBS) and SSc.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


Certificates of Analysis (COA)

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T1503
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25G
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Customers Also Viewed

Reassessing the Role of the Active TGF-?1 as a Biomarker in Systemic Sclerosis: Association of Serum Levels with Clinical Manifestations.
Dantas AT
Disease Markers (2016)
Increased serum concentrations of transforming growth factor-?1 (TGF-?1) in patients with Guillain-Barre syndrome.
Chang KH
Clinica Chimica Acta; International Journal of Clinical Chemistry, 461, 8-13 (2016)
Association between circulating transforming growth factor-?1 level and polymorphisms in systemic lupus erythematosus and rheumatoid arthritis: A meta-analysis.
Lee YH and Bae SC
Cellular and molecular biology (Noisy-le-Grand, France), 63(1), 53-59 (2017)
Qiao Huang et al.
Experimental and therapeutic medicine, 18(1), 692-698 (2019-07-02)
NEF is a tumor suppressing long non-coding (lnc)RNA in hepatocellular carcinoma. Based on current literature, the involvement of NEF in other human diseases is still unknown. The current study aimed to investigate the potential involvement of NEF in glioma, which
Association between Genetic Variants of Transforming Growth Factor-?1 and Susceptibility of Pneumoconiosis: A Meta-analysis.
Deng CW
Chinese Medical Journal (English Edition), 130(3), 357-364 (2017)

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