MilliporeSigma
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RES1161B-A7

SAFC

BIS-TRIS

Synonym(s):
2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
Empirical Formula (Hill Notation):
C8H19NO5
CAS Number:
Molecular Weight:
209.24
Beilstein:
2205275
EC Number:
MDL number:
NACRES:
NA.21

biological source

synthetic

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals, tested

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

H2O: 0.1 M, clear, colorless (aqueous)

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

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This Item
B4429391335B7535
BIS-TRIS

SAFC

RES1161B-A7

BIS-TRIS

BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

Sigma-Aldrich

B4429

BIS-TRIS

BIS-Tris, ULTROL® Grade A zwitterionic buffer that exhibits only a very small shift in dissociation constant with respect to temperature.

Millipore

391335

BIS-Tris, ULTROL® Grade

BIS-TRIS BioXtra, ≥98.0% (titration)

Sigma-Aldrich

B7535

BIS-TRIS

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

impurities

Endotoxin, microbial, and trace metals, tested

impurities

endotoxin and total aerobic microbial count, tested

impurities

-

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

solubility

H2O: 0.1 M, clear, colorless (aqueous)

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

water: soluble

solubility

H2O: 1 M, clear, colorless

suitability

suitable for manufacturing use

suitability

-

suitability

-

suitability

-

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

-

foreign activity

-

foreign activity

-

General description

Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological based formulations.

Bis Tris is used in a wide variety of biological applications including culture media, electrophoresis separations and diagnostic reagent formulations.

Packaging

Product is available in the following package sizes:
RES1161B-A701X: 100g container
RES1161B-A702X 1kg container
RES1161B-A704X: 10kg container
RES1161B-A705X: 25kg container

Legal Information

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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T1503
Product Number
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25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

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Sigma-Aldrich

B6032

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HEPES

BIS-TRIS propane ≥99.0% (titration)

Sigma-Aldrich

B6755

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HEPES

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PHG0001

HEPES

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SAFC

RES0113M-A7

MES monohydrate

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