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RES1161B-A7

SAFC

BIS-TRIS

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Synonym(s):
2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
Empirical Formula (Hill Notation):
C8H19NO5
CAS Number:
6976-37-0
Molecular Weight:
209.24
Beilstein:
2205275
EC Number:
230-237-7
MDL number:
MFCD00002853
NACRES:
NA.21

biological source

synthetic

Quality Level

400

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals, tested

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

H2O: 0.1 M, clear, colorless (aqueous)

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

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This Item
B4429391335B7535
BIS-TRIS

SAFC

RES1161B-A7

BIS-TRIS

BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

Sigma-Aldrich

B4429

BIS-TRIS

BIS-Tris, ULTROL® Grade A zwitterionic buffer that exhibits only a very small shift in dissociation constant with respect to temperature.

Millipore

391335

BIS-Tris, ULTROL® Grade

BIS-TRIS BioXtra, ≥98.0% (titration)

Sigma-Aldrich

B7535

BIS-TRIS

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

impurities

Endotoxin, microbial, and trace metals, tested

impurities

endotoxin and total aerobic microbial count, tested

impurities

-

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

solubility

H2O: 0.1 M, clear, colorless (aqueous)

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

water: soluble

solubility

H2O: 1 M, clear, colorless

suitability

suitable for manufacturing use

suitability

-

suitability

-

suitability

-

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

-

foreign activity

-

foreign activity

-

General description

Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological based formulations.

Bis Tris is used in a wide variety of biological applications including culture media, electrophoresis separations and diagnostic reagent formulations.

Packaging

Product is available in the following package sizes:
RES1161B-A701X: 100g container
RES1161B-A702X 1kg container
RES1161B-A704X: 10kg container
RES1161B-A705X: 25kg container

Legal Information

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318

Precautionary Statements

P280 - P305 + P351 + P338 + P310

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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705578-5MG-PW

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MMYOMAG-74K-13

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BIS-TRIS hydrochloride ≥99.0% (titration)

Sigma-Aldrich

B6032

BIS-TRIS hydrochloride

MES hydrate

SAFC

PHG0003

MES hydrate

MOPS

SAFC

PHG0007

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HEPES

SAFC

RES6003H-B7

HEPES

BIS-TRIS propane ≥99.0% (titration)

Sigma-Aldrich

B6755

BIS-TRIS propane

HEPES

SAFC

PHG0001

HEPES

MES monohydrate

SAFC

RES0113M-A7

MES monohydrate

Alastair W Skeffington et al.
Plant physiology, 165(2), 866-879 (2014-05-02)
The first step on the pathway of starch degradation in Arabidopsis (Arabidopsis thaliana) leaves at night is the phosphorylation of starch polymers, catalyzed by glucan, water dikinase (GWD). It has been suggested that GWD is important for the control of
Zahra Sabouri et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(25), E2567-E2575 (2014-05-14)
The best-understood mechanisms for achieving antibody self/non-self discrimination discard self-reactive antibodies before they can be tested for binding microbial antigens, potentially creating holes in the repertoire. Here we provide evidence for a complementary mechanism: retaining autoantibodies in the repertoire displayed
Lesley Cheng et al.
Kidney international, 86(2), 433-444 (2013-12-20)
Micro RNAs (miRNAs) have been shown to circulate in biological fluids and are enclosed in vesicles such as exosomes; they are present in urine and represent a noninvasive methodology to detect biomarkers for diagnostic testing. The low abundance of RNA
Elizabeth M MacDonald et al.
Disease models & mechanisms, 7(4), 471-481 (2014-02-08)
The purpose of our study was to compare two acquired muscle atrophies and the use of myostatin inhibition for their treatment. Myostatin naturally inhibits skeletal muscle growth by binding to ActRIIB, a receptor on the cell surface of myofibers. Because
X Chen et al.
Oncogene, 33(39), 4724-4734 (2013-10-22)
Uveal melanoma (UM) is a genetically and biologically distinct type of melanoma, and once metastatic there is no effective treatment currently available. Eighty percent of UMs harbor mutations in the Gαq family members GNAQ and GNA11. Understanding the effector pathways
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