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SAB3700420

Sigma-Aldrich

Anti-Swine IgG F(ab′)2-Peroxidase antibody produced in rabbit

affinity isolated antibody, lyophilized powder

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Synonym(s):
Anti-Pig IgG F(ab′)2-Peroxidase antibody produced in rabbit, Anti-Porcine IgG F(ab′)2-Peroxidase antibody produced in rabbit, HRP
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

swine

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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SAB3700442SAB3700434SAB3701083
biological source

rabbit

biological source

rabbit

biological source

goat

biological source

rabbit

technique(s)

immunohistochemistry: suitable, western blot: suitable, indirect ELISA: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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General description

Immunoglobulin G (IgG) is a widely expressed serum antibody. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains.

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Swine IgG F(ab′)2 and Swine Serum. No reaction was seen against Swine IgG F(c) .

Immunogen

Swine IgG F(ab′)2 fragment

Application

Anti-Swine IgG F(ab′)2-Peroxidase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA).(3)

Physical properties

Antibody format: IgG

Physical form

Supplied in 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

pcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Lycopene affects the immune responses of finishing pigs
Marcelise RF, et al.
Italian Journal of Animal Science, 17(3), 666-674 (2018)
Antibody structure, instability, and formulation
Immunobiology: The Immune System in Health and Disease, 96(1), 1-26 (2001)

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