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Key Documents

SAB3500350

Sigma-Aldrich

Anti-β-Actin (ACTB) Antibody

chicken polyclonal

Synonym(s):

Anti-β-actin

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About This Item

MDL number:
MFCD00164531
UNSPSC Code:
12352203
NACRES:
NA.41

Product Name

Anti-β-Actin antibody produced in chicken, affinity isolated antibody, buffered aqueous solution

biological source

chicken

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, human, mouse

technique(s)

indirect ELISA: suitable
western blot: suitable

NCBI accession no.

AAH02409

UniProt accession no.

P60709

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)

General description

The amino-terminal of actin may function as an antigen. The protein is expressed in the cytoplasm. There are about six isoforms of actin identified.

Immunogen

β-actin antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human β-actin.

Application

Anti-β-Actin antibody produced in chicken has been used for Western Blotting.

Biochem/physiol Actions

Actin is a cytoskeletal protein that regulates cell motility, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Mutations in the gene encoding this protein have been associated with Baraitser-Winter cerebrofrontofacial syndrome.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in PBS with 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
50050902

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Localization of isoactins in isolated smooth muscle thin filaments by double gold immunolabeling.
J S Drew
The American Journal of Physiology, 260 (1991)
Tianqi Gong et al.
Molecules (Basel, Switzerland), 26(15) (2021-08-08)
The jumonji domain-containing protein 6 (JMJD6) gene catalyzes the arginine demethylation and lysine hydroxylation of histone and a growing list of its known substrate molecules, including p53 and U2AF65, suggesting a possible role in mRNA splicing and transcription in cancer
Angela Szeto et al.
American journal of physiology. Endocrinology and metabolism, 312(3), E183-E189 (2017-01-05)
It has been demonstrated that the neuropeptide oxytocin (OT) attenuates oxidative stress and inflammation in macrophages. In the current study, we examined the role of inflammation on the expression of the oxytocin receptor (OXTR). We hypothesized that OXTR expression is
A spontaneously immortalized Schwann cell line from aldose reductase-deficient mice as a useful tool for studying polyol pathway and aldehyde metabolism
Naoko Niimi
Journal of Neurochemistry (2017)
?-Actin specifically controls cell growth, migration, and the G-actin pool
Tina M Bunnell
Molecular Biology of the Cell, 22 (2011)
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