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SAB5600204

Sigma-Aldrich

Anti-beta-Actin antibody, Rabbit monoclonal

recombinant, expressed in HEK 293 cells, clone RM112, purified immunoglobulin

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Synonym(s):
ACTB, Actin cytoplastic 1, actin beta, beta-actin

recombinant

expressed in HEK 293 cells

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RM112, monoclonal
recombinant monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

technique(s)

ChIP: 1:200
ELISA: suitable (assay dependent concentration)
flow cytometry: suitable (assay dependent concentration)
immunocytochemistry: 1:200
immunohistochemistry: 1:200
immunoprecipitation (IP): 1:200
western blot: 1:1000

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)

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SAB1305546SAB1305554SAB4502632
antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

clone

RM112, monoclonal, recombinant monoclonal

clone

8H10D10, monoclonal

clone

137CT26.1.1, monoclonal

clone

polyclonal

Gene Information

human ... ACTB(60)

Gene Information

human ... ACTB(60)

Gene Information

human ... ACTB(60)

Gene Information

human ... ACTB(60)

technique(s)

ChIP: 1:200, flow cytometry: suitable (assay dependent concentration), immunohistochemistry: 1:200, western blot: 1:1000, ELISA: suitable (assay dependent concentration), immunoprecipitation (IP): 1:200, immunocytochemistry: 1:200

technique(s)

immunohistochemistry: 1:10-1:50, western blot: 1:2000-1:10000

technique(s)

immunofluorescence: 1:10-1:50, immunohistochemistry: 1:25, western blot: 1:1000

technique(s)

ELISA: 1:10000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

NCBI accession no.

60

NCBI accession no.

NP_001092.1

NCBI accession no.

NP_001092.1

NCBI accession no.

P60709

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Specificity

This antibody reacts to beta-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin.

Immunogen

A peptide corresponding to the N-terminus of beta-actin

Physical form

Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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