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SAB4200084

Sigma-Aldrich

Monoclonal Anti-AGO1 antibody produced in rat

~1 mg/mL, clone 4B8, purified immunoglobulin

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Synonym(s):
Anti-Argonaute-1, Anti-Eukaryotic Translation Initiation Factor 2c, subunit 1, Anti-GERP95, Anti-Golgi Endoplasmic Reticulum protein 95 kDa
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4B8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~95 kDa

species reactivity

human, canine, mouse, monkey, bovine

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using HEK-293T cell extracts

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EIF2C1(26523)
mouse ... Eif2c1(236511)

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SAB4200085SAB4200066WH0027161M1
Gene Information

human ... EIF2C1(26523)
mouse ... Eif2c1(236511)

Gene Information

human ... AGO2(27161)
rat ... Ago2(59117)

Gene Information

human ... EIF2C1(26523)
mouse ... Eif2c1(236511)
rat ... Eif2c1(313594)

Gene Information

human ... EIF2C2(27161)

species reactivity

human, canine, mouse, monkey, bovine

species reactivity

monkey, rat, human, mouse, bovine

species reactivity

human

species reactivity

human

clone

4B8, monoclonal

clone

11A9, monoclonal

clone

polyclonal

clone

2E12-1C9, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

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General description

Monoclonal Anti-AGO1 (rat IgG2a isotype) is derived from the hybridoma 4B8 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a recombinant human AGO1 fusion protein. The Argonaute family of proteins can be subdivided into the Ago subfamily and the Piwi subfamily. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. In human, the Ago subfamily consists of hsAgo1−4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies. A member of this group, Ago1 is also known to be associated with Golgi and with endoplasmic reticulum. The gene is located on chromosome 1. Eukaryotic initiation factor (EIF2C1)/hAgo1 is expressed in low to medium levels in most tissues, but its expression is particularly high in embryonic kidney and lung.

Immunogen

recombinant human AGO1 fusion protein.

Application

  • Monoclonal Anti-AGO1 antibody produced in rat has been used in:
  • western blotting
  • immunoprecipitation
  • chromatin immunoprecipitation(CHIP) assay
  • RNA immunoprecipitation
  • RNA-nChIP experiments

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

Biochem/physiol Actions

The Argonaute proteins are evolutionarily conserved between species and have been implicated in both transcriptional and post-transcriptional gene silencing. Eukaryotic initiation factor (EIF2C1)/argonaute 1 plays a vital role in activation of transcriptional enhancers and also regulates alternative splicing in human cells. EIF2C1 levels are also increased in tumors that lack the Wilm′s tumor suppressor gene WT1.The encoded protein interacts with hypoxia-responsive microRNAs (HRMs) and stimulate angiogenic pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mechanism of allele-selective inhibition of huntingtin expression by duplex RNAs that target CAG repeats: function through the RNAi pathway
Hu J, et al.
Nucleic Acids Research, 40(22), 11270-11280 (2012)
The WEE1 regulators CPEB1 and miR-15b switch from inhibitor to activators at G2/M
Kratassiouk G, et al.
Cell Cycle, 15(5), 667-677 (2016)
RNA activating-double stranded RNA targeting flt-1 promoter inhibits endothelial cell proliferation through soluble FLT-1 upregulation
Choi S, et al.
Testing, 13(3), e0193590-e0193590 (2018)
Argonaute protein as a linker to command center of physiological processes
Wei K, et al.
Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu, 25(4), 430-430 (2013)
Mariano Alló et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(44), 15622-15629 (2014-10-15)
The roles of Argonaute proteins in cytoplasmic microRNA and RNAi pathways are well established. However, their implication in small RNA-mediated transcriptional gene silencing in the mammalian cell nucleus is less understood. We have recently shown that intronic siRNAs cause chromatin

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