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SAB4200365

Sigma-Aldrich

Anti-PIWIL1 antibody, Mouse monoclonal

clone PIWIL1-2, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-PIWIL1 antibody produced in mouse, Monoclonal Anti-HIWI, Monoclonal Anti-MIWI, Monoclonal Anti-PIWI, Monoclonal Anti-piwi-like 1
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PIWIL1-2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~95 kDa

species reactivity

human, mouse

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin® Peroxidase staining system.
immunoprecipitation (IP): suitable
western blot: 2.5-5.0 μg/mL using whole cell extracts of HEK-293T cells over-expressing PIWIL1.

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PIWIL1(9271)
mouse ... Piwil1(57749)

General description

Monoclonal Anti-PIWIL1 (mouse IgG2b isotype) is derived from the hybridoma piwi-like protein 1 (PIWIL1-2) produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to KLH. PIWIL1 is a member of Piwi subfamily. It is expressed mostly to the germline. The encoded protein consists of the PAZ domain and the PIWI domain. The PIWIL1 gene is located on human chromosome 12q24.33.

Immunogen

synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse PIWIL1.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in:
  • immunofluorescence
  • immunoprecipitation
  • flow cytometry analysis29}
  • immunoblotting
  • immunohistochemistry

Biochem/physiol Actions

Piwi proteins are implicated in epigenetic control of gene expression, transposon silencing and translation regulation. It plays vital roles in the germline development and gametogenesis of many metazoan species.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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