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Key Documents

09-838

Sigma-Aldrich

Anti-Histone H3.3 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Histone H3.3

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100 μG
$622.00

$622.00


Estimated to ship onMarch 27, 2025


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100 μG
$622.00

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

clone:
polyclonal
application:
ChIP
DB
ICC
WB
species reactivity:
mouse, human
technique(s):
ChIP: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
citations:
69

$622.00


Estimated to ship onMarch 27, 2025


Request a Bulk Order

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

technique(s)

ChIP: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

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This Item
06-59904-81705-806-AF555
antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

culture supernatant

antibody form

purified immunoglobulin

species reactivity

mouse, human

species reactivity

mouse, rat, human

species reactivity

human

species reactivity

human

clone

polyclonal

clone

polyclonal

clone

MC463, monoclonal

clone

3H10, monoclonal

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

UniProt accession no.

P84243

UniProt accession no.

Q16695

UniProt accession no.

Q16695

UniProt accession no.

Q16695

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.

Specificity

Broad species cross-reactivity expected based on 100% sequence homology.

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H3.3.

Application

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Histone H3.3 in HeLa cells.
Image courtesy of Simon Elsässer of David Allis′ Laboratory, Rockefeller University, New York.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP.
Image courtesy of Simon Elsässer of David Allis′ Laboratory, Rockefeller University, New York.

Dot Blot Analysis: A representative lot was used by an independent laboratory in DB.
Reported by Simon Elsässer, Laboratory of Dr. David Allis, Rockefeller University, New York.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-Histone H3.3 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, DB, ChIP to detect Histone H3.3 also known as Histone H3.3.

Quality

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3.3 on 10 µg of HeLa acid extract.

Target description

~17 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa acid extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


  • Certificates of Analysis (COA)

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    Transcription-coupled recruitment of human CHD1 and CHD2 influences chromatin accessibility and histone H3 and H3.3 occupancy at active chromatin regions.
    Siggens, L; Cordeddu, L; Ronnerblad, M; Lennartsson, A; Ekwall, K
    Epigenetics & Chromatin null
    The octamer is the major form of CENP-A nucleosomes at human centromeres.
    Hasson, D; Panchenko, T; Salimian, KJ; Salman, MU; Sekulic, N; Alonso, A; Warburton, PE; Black, BE
    Nature Structural and Molecular Biology null
    Alyshia Newhart et al.
    The Journal of biological chemistry, 288(27), 19882-19899 (2013-05-22)
    Unlike the core histones, which are incorporated into nucleosomes concomitant with DNA replication, histone H3.3 is synthesized throughout the cell cycle and utilized for replication-independent (RI) chromatin assembly. The RI incorporation of H3.3 into nucleosomes is highly conserved and occurs
    Hsiao P J Voon et al.
    Cell reports, 11(3), 405-418 (2015-04-14)
    Histone H3.3 is a replication-independent histone variant, which replaces histones that are turned over throughout the entire cell cycle. H3.3 deposition at euchromatin is dependent on HIRA, whereas ATRX/Daxx deposits H3.3 at pericentric heterochromatin and telomeres. The role of H3.3
    Egr-1 induces DARPP-32 expression in striatal medium spiny neurons via a conserved intragenic element.
    Keilani, S; Chandwani, S; Dolios, G; Bogush, A; Beck, H; Hatzopoulos, AK; Rao, GN; Thomas et al.
    The Journal of Neuroscience null

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