SAB4200468
Anti-JAM-A antibody, Mouse monoclonal
clone J10.4, purified from hybridoma cell culture
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Synonym(s):
Monoclonal Anti-CD321, Monoclonal Anti-F11 receptor, Monoclonal Anti-F11R, Monoclonal Anti-JAM, Monoclonal Anti-JAM1, Monoclonal Anti-JAMA, Monoclonal Anti-JCAM, Monoclonal Anti-KAT, Monoclonal Anti-PAM-1
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
J10.4, monoclonal
form
buffered aqueous solution
mol wt
antigen 37 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 1.0-2.0 μg/mL using HepG2 or A431 or A549 total cell extracts
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... F11R(50848)
Related Categories
General description
Anti-JAM-A antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma J10.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human JAM. Junctional adhesion molecule A (JAM-A) is expressed at tight junctions of endothelial and epithelial cells as well as on a variety of hematopoietic cells. It belongs to the transmembrane IgG glycoprotein family and is also expressed in monocytes, lymphocytes, neutrophils, dendritic cells, platelets, and spermatozoa. The JAM-A or F11R gene is mapped to human chromosome 1q23.3.
Specificity
Anti-JAM-A antibody, Mouse monoclonal recognizes human JAM-A.
Immunogen
derived from the hybridoma J10.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human JAM fusion protein.
Application
Anti-JAM-A antibody, Mouse monoclonal has been used in:
- western blotting
- staining for confocal microscopy
- immunoprecipitation
- immunocytochemistry
- flow cytometry
Biochem/physiol Actions
Junctional adhesion molecule A(JAM-A) function has been linked with regulation of cell migration/invasion, platelet adhesion, cell polarity, cell proliferation, and epithelial or endothelial barrier function. However, the mechanisms by which JAM-A mediates these processes have not been well elucidated. JAM-A functional significance is connected with inflammation, angiogenesis, hypertension, ischemia/reperfusion, and atherosclerosis. JAM-A has also been linked to breast, endometrial, renal cell carcinomas and gastric cancer.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Hisato Koshiba et al.
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, 19(2), 208-213 (2009-04-28)
Junctional adhesion molecule A (JAM-A) is involved in cell-cell contact and tight junction formation. Loss of cell adhesion molecules may be associated with high histologic grade and invasiveness of endometrial carcinoma. We attempted to determine JAM-A expression in human endometrial
Stefania Vetrano et al.
Annals of the New York Academy of Sciences, 1165, 308-313 (2009-06-23)
Tight junctions (TJ) are junctional proteins whose function is to maintain an intact intestinal epithelial barrier and regulate the paracellular movement of water and solutes. Altered TJ structure and epithelial permeability are observed in inflammatory bowel disease and seem to
Tim Steinbacher et al.
Cellular and molecular life sciences : CMLS, 75(8), 1393-1409 (2017-12-15)
Cell adhesion molecules (CAMs) of the immunoglobulin superfamily (IgSF) regulate important processes such as cell proliferation, differentiation and morphogenesis. This activity is primarily due to their ability to initiate intracellular signaling cascades at cell-cell contact sites. Junctional adhesion molecule-A (JAM-A)
Paul Gutwein et al.
Biochemical and biophysical research communications, 380(2), 387-391 (2009-03-03)
Junctional adhesion molecule-A (JAM-A) is one component of tight junctions which are involved in important processes like paracellular permeability, cell polarity, adhesion, migration, and angiogenesis. Here we describe JAM-A expression in distal convoluted tubule, connecting tubule, and in cells of
Axel Haarmann et al.
PloS one, 5(10), e13568-e13568 (2010-11-10)
An inducible release of soluble junctional adhesion molecule-A (sJAM-A) under pro-inflammatory conditions was described in cultured non-CNS endothelial cells (EC) and increased sJAM-A serum levels were found to indicate inflammation in non-CNS vascular beds. Here we studied the regulation of
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